Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa
In this study, we focused the effects of crebanine, an alkaloid isolated from the tuber of Stephania venosa, on various human cancer cells. Crebanine treatment was found to significantly inhibit the proliferation of human leukemic cells (HL-60, U937 and K562), human fibrosarcoma cells (HT1080) and c...
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th-cmuir.6653943832-39492014-08-30T02:35:30Z Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa Wongsirisin P. Yodkeeree S. Pompimon W. Limtrakul P. In this study, we focused the effects of crebanine, an alkaloid isolated from the tuber of Stephania venosa, on various human cancer cells. Crebanine treatment was found to significantly inhibit the proliferation of human leukemic cells (HL-60, U937 and K562), human fibrosarcoma cells (HT1080) and cervix cancer cell lines (KB-3-1 and KB-V1), of which HL-60 cells were the most sensitive to its treatment. In contrast, crebanine caused much less toxicity in human normal fibroblast cells. Our results demonstrated that crebanine mediated cell cycle arrest at G0/G1 phase and this was associated with down-regulation of cyclins A and D. In addition, crebanine induced apoptosis, which was detected by observation of the membrane phospholipid exposure in flow cytometry. Its induction of apoptosis was accompanied by an increase in cleavage of caspase-3, -8, -9 and poly(ADP-ribose) polymerase (PARP), and was attributable to the augmentation of Bax/Bcl proteins level. Crebanine also decreased mitochondrial membrane potential. Taken together, crebanine exerts anti-proliferative effects on human cancer cells through the induction of cell cycle arrest at the G1 phases and apoptosis. Our results suggest that crebanine is a promising new candidate as a chemotherapeutic agent for cancer therapy. © 2012 The Pharmaceutical Society of Japan. 2014-08-30T02:35:30Z 2014-08-30T02:35:30Z 2012 Article 92363 10.1248/cpb.c12-00506 CPBTA http://www.scopus.com/inward/record.url?eid=2-s2.0-84867196836&partnerID=40&md5=7e04dee3e23da8449c9877e9a1cd984e http://cmuir.cmu.ac.th/handle/6653943832/3949 English |
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In this study, we focused the effects of crebanine, an alkaloid isolated from the tuber of Stephania venosa, on various human cancer cells. Crebanine treatment was found to significantly inhibit the proliferation of human leukemic cells (HL-60, U937 and K562), human fibrosarcoma cells (HT1080) and cervix cancer cell lines (KB-3-1 and KB-V1), of which HL-60 cells were the most sensitive to its treatment. In contrast, crebanine caused much less toxicity in human normal fibroblast cells. Our results demonstrated that crebanine mediated cell cycle arrest at G0/G1 phase and this was associated with down-regulation of cyclins A and D. In addition, crebanine induced apoptosis, which was detected by observation of the membrane phospholipid exposure in flow cytometry. Its induction of apoptosis was accompanied by an increase in cleavage of caspase-3, -8, -9 and poly(ADP-ribose) polymerase (PARP), and was attributable to the augmentation of Bax/Bcl proteins level. Crebanine also decreased mitochondrial membrane potential. Taken together, crebanine exerts anti-proliferative effects on human cancer cells through the induction of cell cycle arrest at the G1 phases and apoptosis. Our results suggest that crebanine is a promising new candidate as a chemotherapeutic agent for cancer therapy. © 2012 The Pharmaceutical Society of Japan. |
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Article |
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Wongsirisin P. Yodkeeree S. Pompimon W. Limtrakul P. |
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Wongsirisin P. Yodkeeree S. Pompimon W. Limtrakul P. Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa |
author_facet |
Wongsirisin P. Yodkeeree S. Pompimon W. Limtrakul P. |
author_sort |
Wongsirisin P. |
title |
Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa |
title_short |
Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa |
title_full |
Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa |
title_fullStr |
Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa |
title_full_unstemmed |
Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from Stephania venosa |
title_sort |
induction of g1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from stephania venosa |
publishDate |
2014 |
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http://www.scopus.com/inward/record.url?eid=2-s2.0-84867196836&partnerID=40&md5=7e04dee3e23da8449c9877e9a1cd984e http://cmuir.cmu.ac.th/handle/6653943832/3949 |
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1681420145129422848 |