Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor

Biopharmaceutical production programs have been established using fed-batch cell culture protocols, which can support high volumetric productivity while providing low operational complexity. The industry is concerned with developing high-titer cell culture processes to meet increasing market demands...

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Main Author:	Porntip Paopang
Other Authors:	Asst. Prof. Dr. Bordin Butr-Indr
Format:	Theses and Dissertations
Language:	English
Published:	เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่ 2017
Subjects:	HIV (Viruses)
Online Access:	http://cmuir.cmu.ac.th/jspui/handle/6653943832/39942
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Institution:	Chiang Mai University
Language:	English

id	th-cmuir.6653943832-39942
record_format	dspace
spelling	th-cmuir.6653943832-399422017-08-24T20:04:40Z Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor การปรับปรุงอาหารเลี้ยงเชื้อแบบกึ่งกะเพื่อผลิตซิงเกิลเชน แวริเอเบิลแฟรกเมนต์ แบบคัดหลั่งต่อโปรตีน p17 ของเชื้อเอชไอวี-1 จากเชื้อเอชเชอริเชีย โคไล ในถังชีวปฏิกรณ์โดยใช้การทดลองแบบแฟคทอเรียลสองวิธีร่วมกัน Porntip Paopang Asst. Prof. Dr. Bordin Butr-Indr Prof.Dr. Chatchai Tayapiwatana Asst. Prof. Dr. Phisit Seesuriyachan HIV (Viruses) Biopharmaceutical production programs have been established using fed-batch cell culture protocols, which can support high volumetric productivity while providing low operational complexity. The industry is concerned with developing high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs. Although advancements in bacterial cell engineering have enabled the development of high-performing recombinant cell lines, developments in cell culture media and process parameter settings are required to realize the maximum production of those cells. We investigated two combined optimization methods, Packlett-Burman design and Sequential Simplex Optimization, for optimization of feed medium. The new combined optimization method decreased the time required by 40.7% compared to the experiment trial. Dry cell weight was 1.24 times higher than in the individual method. The fed-batch cultivation with an optimal feeding rate of 20 ml/h increased cell growth, total protein production and scFv anti-p17 activity by 4.43, 1.48 and 6.5 times compared to batch cultivation respectively. 2017-08-24T04:26:30Z 2017-08-24T04:26:30Z 2014-04-10 Thesis http://cmuir.cmu.ac.th/jspui/handle/6653943832/39942 en เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
language	English
topic	HIV (Viruses)
spellingShingle	HIV (Viruses) Porntip Paopang Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor
description	Biopharmaceutical production programs have been established using fed-batch cell culture protocols, which can support high volumetric productivity while providing low operational complexity. The industry is concerned with developing high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs. Although advancements in bacterial cell engineering have enabled the development of high-performing recombinant cell lines, developments in cell culture media and process parameter settings are required to realize the maximum production of those cells. We investigated two combined optimization methods, Packlett-Burman design and Sequential Simplex Optimization, for optimization of feed medium. The new combined optimization method decreased the time required by 40.7% compared to the experiment trial. Dry cell weight was 1.24 times higher than in the individual method. The fed-batch cultivation with an optimal feeding rate of 20 ml/h increased cell growth, total protein production and scFv anti-p17 activity by 4.43, 1.48 and 6.5 times compared to batch cultivation respectively.
author2	Asst. Prof. Dr. Bordin Butr-Indr
author_facet	Asst. Prof. Dr. Bordin Butr-Indr Porntip Paopang
format	Theses and Dissertations
author	Porntip Paopang
author_sort	Porntip Paopang
title	Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor
title_short	Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor
title_full	Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor
title_fullStr	Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor
title_full_unstemmed	Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor
title_sort	fed-batch medium modification for secreted single chain variable fragment against hiv-1 p17 protein production using combined two factorial experiments in escherichia coli bioreactor
publisher	เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
publishDate	2017
url	http://cmuir.cmu.ac.th/jspui/handle/6653943832/39942
_version_	1681421727259688960

Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor

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