Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa)

In this study, we focused the effects of crebanine, an alkaloid isolated from the tuber of Stephania venosa, on various human cancer cells. Crebanine treatment was found to significantly inhibit the proliferation of human leukemic cells (HL-60, U937 and K562), human fibrosarcoma cells (HT1080) and c...

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Main Authors: Wongsirisin P., Yodkeeree S., Pompimon W., Limtrakul P.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/22863844
http://cmuir.cmu.ac.th/handle/6653943832/4009
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-40092014-08-30T02:35:34Z Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa) Wongsirisin P. Yodkeeree S. Pompimon W. Limtrakul P. In this study, we focused the effects of crebanine, an alkaloid isolated from the tuber of Stephania venosa, on various human cancer cells. Crebanine treatment was found to significantly inhibit the proliferation of human leukemic cells (HL-60, U937 and K562), human fibrosarcoma cells (HT1080) and cervix cancer cell lines (KB-3-1 and KB-V1), of which HL-60 cells were the most sensitive to its treatment. In contrast, crebanine caused much less toxicity in human normal fibroblast cells. Our results demonstrated that crebanine mediated cell cycle arrest at G0/G1 phase and this was associated with down-regulation of cyclin A and D. In addition, crebanine induced apoptosis, which was detected by observation of the membrane phospholipid exposure in flow cytometry. Its induction of apoptosis was accompanied by an increase in cleavage of caspase-3, 8, 9 and poly (ADP-ribose) polymerase, and was attributable to the augmentation of Bax/Bcl proteins level. Crebanine also decreased mitochondrial membrane potential. Taken together, crebanine exerts anti-proliferative effects on human cancer cells through the induction of cell cycle arrest at the G1 phases and apoptosis. Our results suggest that crebanine is a promising new candidate as a chemotherapeutic agent for cancer therapy. 2014-08-30T02:35:34Z 2014-08-30T02:35:34Z 2012 JOURNAL ARTICLE 1347-5223 22863844 http://www.ncbi.nlm.nih.gov/pubmed/22863844 http://cmuir.cmu.ac.th/handle/6653943832/4009 ENG
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description In this study, we focused the effects of crebanine, an alkaloid isolated from the tuber of Stephania venosa, on various human cancer cells. Crebanine treatment was found to significantly inhibit the proliferation of human leukemic cells (HL-60, U937 and K562), human fibrosarcoma cells (HT1080) and cervix cancer cell lines (KB-3-1 and KB-V1), of which HL-60 cells were the most sensitive to its treatment. In contrast, crebanine caused much less toxicity in human normal fibroblast cells. Our results demonstrated that crebanine mediated cell cycle arrest at G0/G1 phase and this was associated with down-regulation of cyclin A and D. In addition, crebanine induced apoptosis, which was detected by observation of the membrane phospholipid exposure in flow cytometry. Its induction of apoptosis was accompanied by an increase in cleavage of caspase-3, 8, 9 and poly (ADP-ribose) polymerase, and was attributable to the augmentation of Bax/Bcl proteins level. Crebanine also decreased mitochondrial membrane potential. Taken together, crebanine exerts anti-proliferative effects on human cancer cells through the induction of cell cycle arrest at the G1 phases and apoptosis. Our results suggest that crebanine is a promising new candidate as a chemotherapeutic agent for cancer therapy.
format Article
author Wongsirisin P.
Yodkeeree S.
Pompimon W.
Limtrakul P.
spellingShingle Wongsirisin P.
Yodkeeree S.
Pompimon W.
Limtrakul P.
Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa)
author_facet Wongsirisin P.
Yodkeeree S.
Pompimon W.
Limtrakul P.
author_sort Wongsirisin P.
title Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa)
title_short Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa)
title_full Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa)
title_fullStr Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa)
title_full_unstemmed Induction of G1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (Stephania venosa)
title_sort induction of g1 arrest and apoptosis in human cancer cells by crebanine, an alkaloid from (stephania venosa)
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/22863844
http://cmuir.cmu.ac.th/handle/6653943832/4009
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