Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes

Enhancement of transdermal absorption through rat skin and stability of the human tyrosinase plasmid (P) using Tat (T) and an entrapment in elastic cationic niosomes (E) were described. E (Tween61:cholesterol:DDAB at 1:1:0.5 molar ratio) were prepared by the freeze-dried empty liposomes (FDELs) meth...

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Main Authors: Manosroi J., Khositsuntiwong N., Manosroi W., Gotz F., Werner R.G., Manosroi A.
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Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84872247846&partnerID=40&md5=1cee3af5a2441b7268839704349ce054
http://cmuir.cmu.ac.th/handle/6653943832/4014
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spelling th-cmuir.6653943832-40142014-08-30T02:35:34Z Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes Manosroi J. Khositsuntiwong N. Manosroi W. Gotz F. Werner R.G. Manosroi A. Enhancement of transdermal absorption through rat skin and stability of the human tyrosinase plasmid (P) using Tat (T) and an entrapment in elastic cationic niosomes (E) were described. E (Tween61:cholesterol:DDAB at 1:1:0.5 molar ratio) were prepared by the freeze-dried empty liposomes (FDELs) method using 25% ethanol. TP was prepared by a simple mixing method. TPE was prepared by loading T and P in E at the T:P:E ratio of 0.5:1:160 w/w/w. For gel formulations, P, TP, PE and TPE were incorporated into Carbopol 980 gel (30 g of plasmid per 1 g of gel). For the transdermal absorption studies, the highest cumulative amounts and fluxes of the plasmid in viable epidermis and dermis (VED) were observed from the TPE of 0.31 ± 0.04 g/cm and 1.86 ± 0.24 g/cm/h (TPE solution); and 4.29 ± 0.40 g/cm and 25.73 ± 2.40 g/cm/h (TPE gel), respectively. Only plasmid from the PE and TPE could be found in the receiving solution with the cumulative amounts and fluxes at 6 h of 0.07 ± 0.01 g/cm and 0.40 ± 0.08 g/cm/h (PE solution); 0.10 ± 0.01 g/cm and 0.60 ± 0.06 g/cm/h (TPE solution); 0.88 ± 0.03 g/cm and 5.30 ± 0.15 g/cm/h (PE gel); and 1.02 ± 0.05 g/cm and 6.13 ± 0.28 g/cm/h (TPE gel), respectively. In stability studies, the plasmid still remained at 4 ± 2 °C and 25 ± 2 °C of about 48.00-65.20% and 27.40-51.10% (solution); and 12.34-38.31% and 8.63-36.10% (gel), respectively, whereas at 45 ± 2 °C, almost all the plasmid was degraded. These studies indicated the high potential application of Tat and an entrapment in elastic cationic niosomes for the development of transdermal gene delivery system. © 2013 Informa Healthcare USA, Inc. 2014-08-30T02:35:34Z 2014-08-30T02:35:34Z 2013 Article 10717544 10.3109/10717544.2012.742937 23311648 DDELE http://www.scopus.com/inward/record.url?eid=2-s2.0-84872247846&partnerID=40&md5=1cee3af5a2441b7268839704349ce054 http://cmuir.cmu.ac.th/handle/6653943832/4014 English
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description Enhancement of transdermal absorption through rat skin and stability of the human tyrosinase plasmid (P) using Tat (T) and an entrapment in elastic cationic niosomes (E) were described. E (Tween61:cholesterol:DDAB at 1:1:0.5 molar ratio) were prepared by the freeze-dried empty liposomes (FDELs) method using 25% ethanol. TP was prepared by a simple mixing method. TPE was prepared by loading T and P in E at the T:P:E ratio of 0.5:1:160 w/w/w. For gel formulations, P, TP, PE and TPE were incorporated into Carbopol 980 gel (30 g of plasmid per 1 g of gel). For the transdermal absorption studies, the highest cumulative amounts and fluxes of the plasmid in viable epidermis and dermis (VED) were observed from the TPE of 0.31 ± 0.04 g/cm and 1.86 ± 0.24 g/cm/h (TPE solution); and 4.29 ± 0.40 g/cm and 25.73 ± 2.40 g/cm/h (TPE gel), respectively. Only plasmid from the PE and TPE could be found in the receiving solution with the cumulative amounts and fluxes at 6 h of 0.07 ± 0.01 g/cm and 0.40 ± 0.08 g/cm/h (PE solution); 0.10 ± 0.01 g/cm and 0.60 ± 0.06 g/cm/h (TPE solution); 0.88 ± 0.03 g/cm and 5.30 ± 0.15 g/cm/h (PE gel); and 1.02 ± 0.05 g/cm and 6.13 ± 0.28 g/cm/h (TPE gel), respectively. In stability studies, the plasmid still remained at 4 ± 2 °C and 25 ± 2 °C of about 48.00-65.20% and 27.40-51.10% (solution); and 12.34-38.31% and 8.63-36.10% (gel), respectively, whereas at 45 ± 2 °C, almost all the plasmid was degraded. These studies indicated the high potential application of Tat and an entrapment in elastic cationic niosomes for the development of transdermal gene delivery system. © 2013 Informa Healthcare USA, Inc.
format Article
author Manosroi J.
Khositsuntiwong N.
Manosroi W.
Gotz F.
Werner R.G.
Manosroi A.
spellingShingle Manosroi J.
Khositsuntiwong N.
Manosroi W.
Gotz F.
Werner R.G.
Manosroi A.
Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes
author_facet Manosroi J.
Khositsuntiwong N.
Manosroi W.
Gotz F.
Werner R.G.
Manosroi A.
author_sort Manosroi J.
title Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes
title_short Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes
title_full Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes
title_fullStr Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes
title_full_unstemmed Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes
title_sort potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pah7/tyr) by tat peptide and an entrapment in elastic cationic niosomes
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84872247846&partnerID=40&md5=1cee3af5a2441b7268839704349ce054
http://cmuir.cmu.ac.th/handle/6653943832/4014
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