Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts

A low cytotoxic elastic niosomal formulation loaded with salmon calcitonin was developed. The elastic niosomes were prepared from Tween 61 mixed with cholesterol at various concentrations of the edge activators (sodium cholate (NaC) and sodium deoxycholate (NaDC); 0.25, 0.5, 2.5, 5 and 10% mole) or...

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Main Authors: Manosroi A., Chankhampan C., Ofoghi H., Manosroi W., Manosroi J.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84871593749&partnerID=40&md5=84d5ce2799be743d5344b681ef34bb14
http://cmuir.cmu.ac.th/handle/6653943832/4021
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-40212014-08-30T02:35:35Z Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts Manosroi A. Chankhampan C. Ofoghi H. Manosroi W. Manosroi J. A low cytotoxic elastic niosomal formulation loaded with salmon calcitonin was developed. The elastic niosomes were prepared from Tween 61 mixed with cholesterol at various concentrations of the edge activators (sodium cholate (NaC) and sodium deoxycholate (NaDC); 0.25, 0.5, 2.5, 5 and 10% mole) or ethanol (10-30% v/v). The effects of the niosomal concentrations (5, 10 and 20 mM) and phosphate buffer at pH 7.0 (5, 10, 20 and 30 mM) on the physical characteristics of niosomes were investigated. The 5 mM elastic niosomes in 5 mM phosphate buffer containing calcitonin 0.22 mg/mL gave the highest elasticity (deformability index (DI)) at 6.79 ± 2.03 determined by the extrusion method. The blank elastic niosomes comprised 2.5% mole NaDC, 5% mole NaC or 20% v/v ethanol showed the highest elasticity. The 5% mole NaC elastic niosomes loaded with calcitonin gave the highest DI (21.59 ± 0.91) and percentages of calcitonin entrapment efficiency (60.11 ± 4.98). This study has demonstrated that this NaC elastic niosome did not only reduce the cytotoxicity of the loaded calcitonin but also gave superior cell viability to the ethanolic elastic niosome as well. © The Author(s) 2013. 2014-08-30T02:35:35Z 2014-08-30T02:35:35Z 2013 Article 09603271 10.1177/0960327112454892 23111885 HETOE http://www.scopus.com/inward/record.url?eid=2-s2.0-84871593749&partnerID=40&md5=84d5ce2799be743d5344b681ef34bb14 http://cmuir.cmu.ac.th/handle/6653943832/4021 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A low cytotoxic elastic niosomal formulation loaded with salmon calcitonin was developed. The elastic niosomes were prepared from Tween 61 mixed with cholesterol at various concentrations of the edge activators (sodium cholate (NaC) and sodium deoxycholate (NaDC); 0.25, 0.5, 2.5, 5 and 10% mole) or ethanol (10-30% v/v). The effects of the niosomal concentrations (5, 10 and 20 mM) and phosphate buffer at pH 7.0 (5, 10, 20 and 30 mM) on the physical characteristics of niosomes were investigated. The 5 mM elastic niosomes in 5 mM phosphate buffer containing calcitonin 0.22 mg/mL gave the highest elasticity (deformability index (DI)) at 6.79 ± 2.03 determined by the extrusion method. The blank elastic niosomes comprised 2.5% mole NaDC, 5% mole NaC or 20% v/v ethanol showed the highest elasticity. The 5% mole NaC elastic niosomes loaded with calcitonin gave the highest DI (21.59 ± 0.91) and percentages of calcitonin entrapment efficiency (60.11 ± 4.98). This study has demonstrated that this NaC elastic niosome did not only reduce the cytotoxicity of the loaded calcitonin but also gave superior cell viability to the ethanolic elastic niosome as well. © The Author(s) 2013.
format Article
author Manosroi A.
Chankhampan C.
Ofoghi H.
Manosroi W.
Manosroi J.
spellingShingle Manosroi A.
Chankhampan C.
Ofoghi H.
Manosroi W.
Manosroi J.
Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts
author_facet Manosroi A.
Chankhampan C.
Ofoghi H.
Manosroi W.
Manosroi J.
author_sort Manosroi A.
title Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts
title_short Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts
title_full Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts
title_fullStr Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts
title_full_unstemmed Low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts
title_sort low cytotoxic elastic niosomes loaded with salmon calcitonin on human skin fibroblasts
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84871593749&partnerID=40&md5=84d5ce2799be743d5344b681ef34bb14
http://cmuir.cmu.ac.th/handle/6653943832/4021
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