Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility

Contents: Phospholipase C zeta (PLCz) and cyclooxygenase isoenzyme type 2 (COX-2) are important in spermatogenesis, but their effect has not yet confirmed in pigs. Therefore, this study was aimed to analyse their association with sperm quality and fertility and to identify the mRNA and protein expre...

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Main Authors: Kaewmala K., Uddin M., Cinar M., Grosse-Brinkhaus C., Jonas E., Tesfaye D., Phatsara C., Tholen E., Looft C., Schellander K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-79960202036&partnerID=40&md5=6df892f080761b911a902b9ddf17a074
http://www.ncbi.nlm.nih.gov/pubmed/21752105
http://cmuir.cmu.ac.th/handle/6653943832/404
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spelling th-cmuir.6653943832-4042014-08-29T07:31:43Z Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility Kaewmala K. Uddin M. Cinar M. Grosse-Brinkhaus C. Jonas E. Tesfaye D. Phatsara C. Tholen E. Looft C. Schellander K. Contents: Phospholipase C zeta (PLCz) and cyclooxygenase isoenzyme type 2 (COX-2) are important in spermatogenesis, but their effect has not yet confirmed in pigs. Therefore, this study was aimed to analyse their association with sperm quality and fertility and to identify the mRNA and protein expression in boars reproductive tissues. DNA samples from 231 Pietrain (PI) and 109 Pietrain×Hampshire (PIHA) pigs with records of sperm quality [sperm concentration (SCON), motility, semen volume, plasma droplet and abnormal spermatozoa rate] and fertility (non-return rate and number of piglet born alive) traits were available. A SNP in non-coding region of PLCz g.158 A>C was associated with SCON (p<0.05) in PIHA population while the polymorphism of COX-2 g.68G>A in 3' UTR was not associated with any traits. For mRNA and protein expression study, a total of six boars were divided into two groups with G-I and G-II, where G-I was characterized for relatively better sperm quality. Both genes expressed higher in reproductive tissues compared with non-reproductive tissues. Phospholipase C zeta mRNA expressed higher in testis (p<0.01), all parts of epididymis and spermatozoa from G-I, while COX-2 expressed higher in testis (p<0.05), head and body of epididymis (p<0.01), and spermatozoa from G-II boar. Both proteins were localized in Leydig cells and spermatozoa. These results might shed light on roles of these genes in spermatogenesis as candidate for boar sperm quality and fertility, but still the lack of association across populations should be considered. © 2011 Blackwell Verlag GmbH. 2014-08-29T07:31:43Z 2014-08-29T07:31:43Z Article in Press 9366768 10.1111/j.1439-0531.2011.01831.x RDANE http://www.scopus.com/inward/record.url?eid=2-s2.0-79960202036&partnerID=40&md5=6df892f080761b911a902b9ddf17a074 http://www.ncbi.nlm.nih.gov/pubmed/21752105 http://cmuir.cmu.ac.th/handle/6653943832/404 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
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language English
description Contents: Phospholipase C zeta (PLCz) and cyclooxygenase isoenzyme type 2 (COX-2) are important in spermatogenesis, but their effect has not yet confirmed in pigs. Therefore, this study was aimed to analyse their association with sperm quality and fertility and to identify the mRNA and protein expression in boars reproductive tissues. DNA samples from 231 Pietrain (PI) and 109 Pietrain×Hampshire (PIHA) pigs with records of sperm quality [sperm concentration (SCON), motility, semen volume, plasma droplet and abnormal spermatozoa rate] and fertility (non-return rate and number of piglet born alive) traits were available. A SNP in non-coding region of PLCz g.158 A>C was associated with SCON (p<0.05) in PIHA population while the polymorphism of COX-2 g.68G>A in 3' UTR was not associated with any traits. For mRNA and protein expression study, a total of six boars were divided into two groups with G-I and G-II, where G-I was characterized for relatively better sperm quality. Both genes expressed higher in reproductive tissues compared with non-reproductive tissues. Phospholipase C zeta mRNA expressed higher in testis (p<0.01), all parts of epididymis and spermatozoa from G-I, while COX-2 expressed higher in testis (p<0.05), head and body of epididymis (p<0.01), and spermatozoa from G-II boar. Both proteins were localized in Leydig cells and spermatozoa. These results might shed light on roles of these genes in spermatogenesis as candidate for boar sperm quality and fertility, but still the lack of association across populations should be considered. © 2011 Blackwell Verlag GmbH.
format Article
author Kaewmala K.
Uddin M.
Cinar M.
Grosse-Brinkhaus C.
Jonas E.
Tesfaye D.
Phatsara C.
Tholen E.
Looft C.
Schellander K.
spellingShingle Kaewmala K.
Uddin M.
Cinar M.
Grosse-Brinkhaus C.
Jonas E.
Tesfaye D.
Phatsara C.
Tholen E.
Looft C.
Schellander K.
Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
author_facet Kaewmala K.
Uddin M.
Cinar M.
Grosse-Brinkhaus C.
Jonas E.
Tesfaye D.
Phatsara C.
Tholen E.
Looft C.
Schellander K.
author_sort Kaewmala K.
title Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
title_short Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
title_full Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
title_fullStr Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
title_full_unstemmed Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
title_sort investigation into association and expression of plcz and cox-2 as candidate genes for boar sperm quality and fertility
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-79960202036&partnerID=40&md5=6df892f080761b911a902b9ddf17a074
http://www.ncbi.nlm.nih.gov/pubmed/21752105
http://cmuir.cmu.ac.th/handle/6653943832/404
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