Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture

Cephalosporins (CEFs) are antibiotics frequently used to treat bone infections and septic arthritis. The effects of CEFs on chondrocytes have not been studied until now. Cefazolin (cef1) and ceftriaxone (cef3), first-and third-generation CEFs, were selected to investigate their direct effects on nor...

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Main Authors: Siengdee P., Pradit W., Euppayo T., Chomdej S., Nganvongpanit K.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85017382859&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/40902
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-409022017-09-28T04:14:27Z Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture Siengdee P. Pradit W. Euppayo T. Chomdej S. Nganvongpanit K. Cephalosporins (CEFs) are antibiotics frequently used to treat bone infections and septic arthritis. The effects of CEFs on chondrocytes have not been studied until now. Cefazolin (cef1) and ceftriaxone (cef3), first-and third-generation CEFs, were selected to investigate their direct effects on normal and osteoarthritic (OA) primary canine chondrocytes, which were either nonstimulated or stimulated with the pro-inflammatory cytokine IL-1β. In our results, treatment with CEFs increased the negative effects on both conditioned normal and OA chondrocytes, especially when applied to IL-1β-stimulated cells (inflammatory stimulus). CEFs significantly decreased cell viability and induced apoptotic cell death in both normal and OA chondrocytes; moreover, treatment with cef1 caused necrotic cell death in OA chondrocytes. Cef3 treatment could increase s-GAG synthesis in normal cells preincubated with IL-1β, while cef1 had no significant effect. The expression of TNF was clearly downregulated after cef3 treatments, whereas it was upregulated after cef1 treatments. However, cef3 induced stronger downregulation of TIMP1 and the extracellular matrix component genes COL2A1 and ACAN. In conclusion, these results suggest both the cytotoxic effects of CEFs and their adverse effects on chondrogenic marker genes at the transcriptional level, which provide additional insight into the clinical application of cef1 and cef3. 2017 John Wiley & Sons Ltd. 2017-09-28T04:14:27Z 2017-09-28T04:14:27Z 2017-01-01 Journal 01407783 2-s2.0-85017382859 10.1111/jvp.12401 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85017382859&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/40902
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Cephalosporins (CEFs) are antibiotics frequently used to treat bone infections and septic arthritis. The effects of CEFs on chondrocytes have not been studied until now. Cefazolin (cef1) and ceftriaxone (cef3), first-and third-generation CEFs, were selected to investigate their direct effects on normal and osteoarthritic (OA) primary canine chondrocytes, which were either nonstimulated or stimulated with the pro-inflammatory cytokine IL-1β. In our results, treatment with CEFs increased the negative effects on both conditioned normal and OA chondrocytes, especially when applied to IL-1β-stimulated cells (inflammatory stimulus). CEFs significantly decreased cell viability and induced apoptotic cell death in both normal and OA chondrocytes; moreover, treatment with cef1 caused necrotic cell death in OA chondrocytes. Cef3 treatment could increase s-GAG synthesis in normal cells preincubated with IL-1β, while cef1 had no significant effect. The expression of TNF was clearly downregulated after cef3 treatments, whereas it was upregulated after cef1 treatments. However, cef3 induced stronger downregulation of TIMP1 and the extracellular matrix component genes COL2A1 and ACAN. In conclusion, these results suggest both the cytotoxic effects of CEFs and their adverse effects on chondrogenic marker genes at the transcriptional level, which provide additional insight into the clinical application of cef1 and cef3. 2017 John Wiley & Sons Ltd.
format Journal
author Siengdee P.
Pradit W.
Euppayo T.
Chomdej S.
Nganvongpanit K.
spellingShingle Siengdee P.
Pradit W.
Euppayo T.
Chomdej S.
Nganvongpanit K.
Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture
author_facet Siengdee P.
Pradit W.
Euppayo T.
Chomdej S.
Nganvongpanit K.
author_sort Siengdee P.
title Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture
title_short Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture
title_full Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture
title_fullStr Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture
title_full_unstemmed Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture
title_sort comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85017382859&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/40902
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