Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers

Background: It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.Methods: In exp...

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Main Authors: Chailert C., Sanmee U., Piromlertamorn W., Samchimchom S., Vutyavanich T.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/23510434
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spelling th-cmuir.6653943832-40932014-08-30T02:35:40Z Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers Chailert C. Sanmee U. Piromlertamorn W. Samchimchom S. Vutyavanich T. Background: It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.Methods: In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the zona pellucida with an infrared laser beam. In the second experiment, 0, 1 and 2 openings of 20 microns were studied. In the third experiment, a full-thickness opening of 20 microns or quarter-thinning of the zonal circumference to a depth of 90% was compared with non-AH controls.Results: No difference in blastocyst formation was found in laser-treated groups and in the controls. In experiment 1, the rate of completely hatched blastocysts was significantly lower than the controls. In experiment 2 when the size of the opening was increased, blastocysts completely hatched at a significantly higher rate than that in the controls. In experiment 3, the rate of completely hatched blastocysts was the highest in the full-thickness group. Cell numbers in completely hatched blastocysts from both AH groups were significantly fewer than those in the controls.Conclusions: Full-thickness opening resulted in a higher rate of completely hatched blastocysts than quarter zonal-thinning and controls, but the cell numbers were significantly decreased. © 2013 Chailert et al.; licensee BioMed Central Ltd. 2014-08-30T02:35:40Z 2014-08-30T02:35:40Z 2013 Article 14777827 10.1186/1477-7827-11-21 23510434 http://www.ncbi.nlm.nih.gov/pubmed/23510434 http://www.scopus.com/inward/record.url?eid=2-s2.0-84875040166&partnerID=40&md5=d59405c56168dee62f2fad3d742da56d http://cmuir.cmu.ac.th/handle/6653943832/4093 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Background: It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.Methods: In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the zona pellucida with an infrared laser beam. In the second experiment, 0, 1 and 2 openings of 20 microns were studied. In the third experiment, a full-thickness opening of 20 microns or quarter-thinning of the zonal circumference to a depth of 90% was compared with non-AH controls.Results: No difference in blastocyst formation was found in laser-treated groups and in the controls. In experiment 1, the rate of completely hatched blastocysts was significantly lower than the controls. In experiment 2 when the size of the opening was increased, blastocysts completely hatched at a significantly higher rate than that in the controls. In experiment 3, the rate of completely hatched blastocysts was the highest in the full-thickness group. Cell numbers in completely hatched blastocysts from both AH groups were significantly fewer than those in the controls.Conclusions: Full-thickness opening resulted in a higher rate of completely hatched blastocysts than quarter zonal-thinning and controls, but the cell numbers were significantly decreased. © 2013 Chailert et al.; licensee BioMed Central Ltd.
format Article
author Chailert C.
Sanmee U.
Piromlertamorn W.
Samchimchom S.
Vutyavanich T.
spellingShingle Chailert C.
Sanmee U.
Piromlertamorn W.
Samchimchom S.
Vutyavanich T.
Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
author_facet Chailert C.
Sanmee U.
Piromlertamorn W.
Samchimchom S.
Vutyavanich T.
author_sort Chailert C.
title Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
title_short Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
title_full Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
title_fullStr Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
title_full_unstemmed Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
title_sort effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/23510434
http://www.scopus.com/inward/record.url?eid=2-s2.0-84875040166&partnerID=40&md5=d59405c56168dee62f2fad3d742da56d
http://cmuir.cmu.ac.th/handle/6653943832/4093
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