Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
Background: It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.Methods: In exp...
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th-cmuir.6653943832-40932014-08-30T02:35:40Z Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers Chailert C. Sanmee U. Piromlertamorn W. Samchimchom S. Vutyavanich T. Background: It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.Methods: In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the zona pellucida with an infrared laser beam. In the second experiment, 0, 1 and 2 openings of 20 microns were studied. In the third experiment, a full-thickness opening of 20 microns or quarter-thinning of the zonal circumference to a depth of 90% was compared with non-AH controls.Results: No difference in blastocyst formation was found in laser-treated groups and in the controls. In experiment 1, the rate of completely hatched blastocysts was significantly lower than the controls. In experiment 2 when the size of the opening was increased, blastocysts completely hatched at a significantly higher rate than that in the controls. In experiment 3, the rate of completely hatched blastocysts was the highest in the full-thickness group. Cell numbers in completely hatched blastocysts from both AH groups were significantly fewer than those in the controls.Conclusions: Full-thickness opening resulted in a higher rate of completely hatched blastocysts than quarter zonal-thinning and controls, but the cell numbers were significantly decreased. © 2013 Chailert et al.; licensee BioMed Central Ltd. 2014-08-30T02:35:40Z 2014-08-30T02:35:40Z 2013 Article 14777827 10.1186/1477-7827-11-21 23510434 http://www.ncbi.nlm.nih.gov/pubmed/23510434 http://www.scopus.com/inward/record.url?eid=2-s2.0-84875040166&partnerID=40&md5=d59405c56168dee62f2fad3d742da56d http://cmuir.cmu.ac.th/handle/6653943832/4093 English |
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Background: It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.Methods: In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the zona pellucida with an infrared laser beam. In the second experiment, 0, 1 and 2 openings of 20 microns were studied. In the third experiment, a full-thickness opening of 20 microns or quarter-thinning of the zonal circumference to a depth of 90% was compared with non-AH controls.Results: No difference in blastocyst formation was found in laser-treated groups and in the controls. In experiment 1, the rate of completely hatched blastocysts was significantly lower than the controls. In experiment 2 when the size of the opening was increased, blastocysts completely hatched at a significantly higher rate than that in the controls. In experiment 3, the rate of completely hatched blastocysts was the highest in the full-thickness group. Cell numbers in completely hatched blastocysts from both AH groups were significantly fewer than those in the controls.Conclusions: Full-thickness opening resulted in a higher rate of completely hatched blastocysts than quarter zonal-thinning and controls, but the cell numbers were significantly decreased. © 2013 Chailert et al.; licensee BioMed Central Ltd. |
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Chailert C. Sanmee U. Piromlertamorn W. Samchimchom S. Vutyavanich T. |
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Chailert C. Sanmee U. Piromlertamorn W. Samchimchom S. Vutyavanich T. Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers |
author_facet |
Chailert C. Sanmee U. Piromlertamorn W. Samchimchom S. Vutyavanich T. |
author_sort |
Chailert C. |
title |
Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers |
title_short |
Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers |
title_full |
Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers |
title_fullStr |
Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers |
title_full_unstemmed |
Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers |
title_sort |
effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pubmed/23510434 http://www.scopus.com/inward/record.url?eid=2-s2.0-84875040166&partnerID=40&md5=d59405c56168dee62f2fad3d742da56d http://cmuir.cmu.ac.th/handle/6653943832/4093 |
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1681420172381913088 |