3D modeling of keloid scars in vitro by cell and tissue engineering

© 2016, Springer-Verlag Berlin Heidelberg. Keloids are pathologic scars defined as dermal fibrotic tumors resulting from a disturbance of skin wound healing process. Treatments against keloids are multiple, sometimes empirical and none of them really provides an effective tool for physicians. The la...

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Main Authors: Suttho D., Mankhetkorn S., Binda D., Pazart L., Humbert P., Rolin G.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85004045171&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41101
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-411012017-09-28T04:15:35Z 3D modeling of keloid scars in vitro by cell and tissue engineering Suttho D. Mankhetkorn S. Binda D. Pazart L. Humbert P. Rolin G. © 2016, Springer-Verlag Berlin Heidelberg. Keloids are pathologic scars defined as dermal fibrotic tumors resulting from a disturbance of skin wound healing process. Treatments against keloids are multiple, sometimes empirical and none of them really provides an effective tool for physicians. The lack of effective treatments is correlated with the poor understanding of keloid pathogenesis. To fill this gap, researchers need strong models mimicking keloids as closely as possible. The objective of this study was to establish in vitro a new reconstructed keloid model (RKM), by combining fibroblasts extracted from the three major area of a keloid (center, periphery, non-lesional) in a three-dimensional biomaterial. To this aim, fibroblasts of three keloid locations were extracted and characterized, and then integrated in a hydrated collagen gel matrix during a three-step procedure. The heterogeneity of fibroblasts was assessed according to their proliferative and remodeling capacities. RKMs were further visualized and characterized by both light and scanning electron microscopy. This reconstructed keloid model should be very useful for investigating keloid fibroblasts function in conditions mimicking in vivo situation. Moreover, RKM should also be a suitable model for either drug study and discovery or innovative approaches using medical devices both during cancer and cancer-like disease investigation. 2017-09-28T04:15:35Z 2017-09-28T04:15:35Z 2017-01-01 Journal 03403696 2-s2.0-85004045171 10.1007/s00403-016-1703-2 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85004045171&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/41101
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016, Springer-Verlag Berlin Heidelberg. Keloids are pathologic scars defined as dermal fibrotic tumors resulting from a disturbance of skin wound healing process. Treatments against keloids are multiple, sometimes empirical and none of them really provides an effective tool for physicians. The lack of effective treatments is correlated with the poor understanding of keloid pathogenesis. To fill this gap, researchers need strong models mimicking keloids as closely as possible. The objective of this study was to establish in vitro a new reconstructed keloid model (RKM), by combining fibroblasts extracted from the three major area of a keloid (center, periphery, non-lesional) in a three-dimensional biomaterial. To this aim, fibroblasts of three keloid locations were extracted and characterized, and then integrated in a hydrated collagen gel matrix during a three-step procedure. The heterogeneity of fibroblasts was assessed according to their proliferative and remodeling capacities. RKMs were further visualized and characterized by both light and scanning electron microscopy. This reconstructed keloid model should be very useful for investigating keloid fibroblasts function in conditions mimicking in vivo situation. Moreover, RKM should also be a suitable model for either drug study and discovery or innovative approaches using medical devices both during cancer and cancer-like disease investigation.
format Journal
author Suttho D.
Mankhetkorn S.
Binda D.
Pazart L.
Humbert P.
Rolin G.
spellingShingle Suttho D.
Mankhetkorn S.
Binda D.
Pazart L.
Humbert P.
Rolin G.
3D modeling of keloid scars in vitro by cell and tissue engineering
author_facet Suttho D.
Mankhetkorn S.
Binda D.
Pazart L.
Humbert P.
Rolin G.
author_sort Suttho D.
title 3D modeling of keloid scars in vitro by cell and tissue engineering
title_short 3D modeling of keloid scars in vitro by cell and tissue engineering
title_full 3D modeling of keloid scars in vitro by cell and tissue engineering
title_fullStr 3D modeling of keloid scars in vitro by cell and tissue engineering
title_full_unstemmed 3D modeling of keloid scars in vitro by cell and tissue engineering
title_sort 3d modeling of keloid scars in vitro by cell and tissue engineering
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85004045171&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41101
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