Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats
Spirogyra neglecta extract (SNE) has shown antihyperglycemia and antihyperlipidemia in type 2 diabetic mellitus (T2DM) rats. This study investigated the antioxidant and renoprotective effects of SNE in T2DM rats induced by high-fat diet with low-single dose streptozotocin. T2DM rats were fed daily w...
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th-cmuir.6653943832-41152014-08-30T02:35:41Z Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats Ontawong A. Saowakon N. Vivithanaporn P. Pongchaidecha A. Lailerd N. Amornlerdpison D. Lungkaphin A. Srimaroeng C. Spirogyra neglecta extract (SNE) has shown antihyperglycemia and antihyperlipidemia in type 2 diabetic mellitus (T2DM) rats. This study investigated the antioxidant and renoprotective effects of SNE in T2DM rats induced by high-fat diet with low-single dose streptozotocin. T2DM rats were fed daily with SNE (0.25, 0.5, and 1 g/kg BW) for 12 weeks. Renal morphology, malondialdehyde levels, qPCR, and western blotting were analyzed. Renal cortical slices were used to determine renal transport of organic anions, which are estrone sulfate and para-aminohippurate, mediated through organic anion transporter 3-Oat3. Insulin and PKC ζ were known to activate Oat3 function while it was inhibited by PKC α. Compared to T2DM, plasma glucose, triglyceride, insulin resistance, renal morphology, and malondialdehyde levels were significantly improved by SNE supplementation. Reduced glutathione peroxidase and nuclear factor B expressions were related to antioxidant effect of SNE. Oat3 mRNA and protein were not different among groups, but insulin-stimulated rOat3 followed by anion uptakes was abolished in T2DM. This was restored in the slices from SNE treatment. The mechanism of SNE-improved Oat3 was associated with PKC α and PKC ζ expressions and activities. These findings indicate that SNE has beneficial effects on renal transport through antioxidant enzymes and PKCs in T2DM rats. © 2013 Atcharaporn Ontawong et al. 2014-08-30T02:35:41Z 2014-08-30T02:35:41Z 2013 Article 23146133 10.1155/2013/820786 23862157 http://www.scopus.com/inward/record.url?eid=2-s2.0-84879348847&partnerID=40&md5=ad65ea5b88a7aa9010bfdf4bd7496408 http://cmuir.cmu.ac.th/handle/6653943832/4115 English |
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Spirogyra neglecta extract (SNE) has shown antihyperglycemia and antihyperlipidemia in type 2 diabetic mellitus (T2DM) rats. This study investigated the antioxidant and renoprotective effects of SNE in T2DM rats induced by high-fat diet with low-single dose streptozotocin. T2DM rats were fed daily with SNE (0.25, 0.5, and 1 g/kg BW) for 12 weeks. Renal morphology, malondialdehyde levels, qPCR, and western blotting were analyzed. Renal cortical slices were used to determine renal transport of organic anions, which are estrone sulfate and para-aminohippurate, mediated through organic anion transporter 3-Oat3. Insulin and PKC ζ were known to activate Oat3 function while it was inhibited by PKC α. Compared to T2DM, plasma glucose, triglyceride, insulin resistance, renal morphology, and malondialdehyde levels were significantly improved by SNE supplementation. Reduced glutathione peroxidase and nuclear factor B expressions were related to antioxidant effect of SNE. Oat3 mRNA and protein were not different among groups, but insulin-stimulated rOat3 followed by anion uptakes was abolished in T2DM. This was restored in the slices from SNE treatment. The mechanism of SNE-improved Oat3 was associated with PKC α and PKC ζ expressions and activities. These findings indicate that SNE has beneficial effects on renal transport through antioxidant enzymes and PKCs in T2DM rats. © 2013 Atcharaporn Ontawong et al. |
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Ontawong A. Saowakon N. Vivithanaporn P. Pongchaidecha A. Lailerd N. Amornlerdpison D. Lungkaphin A. Srimaroeng C. |
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Ontawong A. Saowakon N. Vivithanaporn P. Pongchaidecha A. Lailerd N. Amornlerdpison D. Lungkaphin A. Srimaroeng C. Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats |
author_facet |
Ontawong A. Saowakon N. Vivithanaporn P. Pongchaidecha A. Lailerd N. Amornlerdpison D. Lungkaphin A. Srimaroeng C. |
author_sort |
Ontawong A. |
title |
Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats |
title_short |
Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats |
title_full |
Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats |
title_fullStr |
Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats |
title_full_unstemmed |
Antioxidant and renoprotective effects of Spirogyra neglecta (Hassall) Kützing extract in experimental type 2 diabetic rats |
title_sort |
antioxidant and renoprotective effects of spirogyra neglecta (hassall) kützing extract in experimental type 2 diabetic rats |
publishDate |
2014 |
url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-84879348847&partnerID=40&md5=ad65ea5b88a7aa9010bfdf4bd7496408 http://cmuir.cmu.ac.th/handle/6653943832/4115 |
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