Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease

Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease

© 2016 Taylor & Francis. Monoclonal antibodies against α-globin containing human Hbs, named AMS-Alpha1 and AMS-Alpha 2, were produced by the hybridoma technique using spleen cells enriched by the newly developed B lymphocyte enrichment protocol. These two monoclonal antibodies were of IgM clas...

Full description

Saved in:
Bibliographic Details
Main Authors: Pakdeepak K., Pata S., Chiampanichayakul S., Kasinrerk W., Tatu T.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84987958839&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41383
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-41383
record_format dspace
spelling th-cmuir.6653943832-413832017-09-28T04:20:59Z Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease Pakdeepak K. Pata S. Chiampanichayakul S. Kasinrerk W. Tatu T. © 2016 Taylor & Francis. Monoclonal antibodies against α-globin containing human Hbs, named AMS-Alpha1 and AMS-Alpha 2, were produced by the hybridoma technique using spleen cells enriched by the newly developed B lymphocyte enrichment protocol. These two monoclonal antibodies were of IgM class, reacting to only intact form of human Hbs A, A 2 , E, and F, which contain α-globin chain. By the indirect ELISA, the AMS-Alpha1 and AMS-Alpha 2 quantified less amount of α-globin chain containing hemoglobins in HbH disease than the SEA-α thalassemia 1 carriers and normal individuals. It was thus anticipated that these monoclonal antibodies can be used for detecting Hb Bart’s hydrops fetalis in which no α-globin chain is produced. 2017-09-28T04:20:59Z 2017-09-28T04:20:59Z 2016-11-01 Journal 15321819 2-s2.0-84987958839 10.1080/15321819.2016.1174135 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84987958839&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/41383
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016 Taylor & Francis. Monoclonal antibodies against α-globin containing human Hbs, named AMS-Alpha1 and AMS-Alpha 2, were produced by the hybridoma technique using spleen cells enriched by the newly developed B lymphocyte enrichment protocol. These two monoclonal antibodies were of IgM class, reacting to only intact form of human Hbs A, A 2 , E, and F, which contain α-globin chain. By the indirect ELISA, the AMS-Alpha1 and AMS-Alpha 2 quantified less amount of α-globin chain containing hemoglobins in HbH disease than the SEA-α thalassemia 1 carriers and normal individuals. It was thus anticipated that these monoclonal antibodies can be used for detecting Hb Bart’s hydrops fetalis in which no α-globin chain is produced.
format Journal
author Pakdeepak K.
Pata S.
Chiampanichayakul S.
Kasinrerk W.
Tatu T.
spellingShingle Pakdeepak K.
Pata S.
Chiampanichayakul S.
Kasinrerk W.
Tatu T.
Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
author_facet Pakdeepak K.
Pata S.
Chiampanichayakul S.
Kasinrerk W.
Tatu T.
author_sort Pakdeepak K.
title Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
title_short Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
title_full Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
title_fullStr Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
title_full_unstemmed Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
title_sort production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84987958839&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41383
_version_ 1681421991980040192

Similar Items