Streptomyces palmae sp. nov., isolated from oil palm (Elaeis Guineensis) rhizosphere soil

• Main Authors: Sujarit K., Kudo T., Ohkuma M., Pathom-Aree W., Lumyong S.
• Format: Journal
• Published: 2017
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84994517940&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/41465
• Institution: Chiang Mai University

© 2016 IUMS. Actinomycete strain CMU-AB204 T was isolated from oil palm rhizosphere soil collected in Chiang Mai University (Chiang Mai, Thailand). Based on morphological and chemotaxonomic characteristics, the organism was considered to belong to the genus Streptomyces. Whole cellwall hydrolysates consisted of LL-diaminopimelic acid, glucose, ribose and galactose. The predominant menaquinones were MK-9(H 4 ), MK-9(H 6 ), MK-9(H 2 ) and MK-8(H 4 ). The fatty acid profile contained iso-C 15: 0 , iso-C 16: 0 and anteiso-C15: 0 as major components. The principal phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content of strain CMU-AB204 T was 70.9 mol%. Based on 16S rRNA gene sequence similarity, strain CMU-AB204 T was closely related to Streptomyces orinoci JCM 4546 T (98.7 %), Streptomyces lilacinus NBRC 12884 T (98.5 %), Streptomyces abikoensis CGMCC 4.1662 T (98.5 %), Streptomyces griseocarneus JCM 4905 T (98.4 %) and Streptomyces xinghaiensis JCM 16958 T (98.3 %). Phylogenetic trees revealed that the new strain had a distinct taxonomic position from closely related type strains of the genus Streptomyces. Spiny to hairy spores clearly differentiated strain CMU-AB204 T from the five most closely related Streptomyces species, which produced smooth spores. On the basis of evidence from this polyphasic study, it is proposed that strain CMU-AB204 T represents a novel species of the genus Streptomyces, namely Streptomyces palmae sp. nov. The type strain is CMU-AB204 T (=JCM 31289 T =TBRC 1999 T ).