Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis

Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis

© 2016 Taylor & Francis. The current available assays cannot differentiate the stages of phagocytosis. We, therefore, established methods for concurrent detection of antigen attachment and engulfment by phagocyte using latex beads coated with lipopolysaccharide, rabbit IgG, and carboxyfluoresc...

Full description

Saved in:
Bibliographic Details
Main Authors: Laopajon W., Takheaw N., Kasinrerk W., Pata S.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84975512237&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41547
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-41547
record_format dspace
spelling th-cmuir.6653943832-415472017-09-28T04:21:53Z Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis Laopajon W. Takheaw N. Kasinrerk W. Pata S. © 2016 Taylor & Francis. The current available assays cannot differentiate the stages of phagocytosis. We, therefore, established methods for concurrent detection of antigen attachment and engulfment by phagocyte using latex beads coated with lipopolysaccharide, rabbit IgG, and carboxyfluorescein diacetate succinimidyl ester. The generated beads were incubated with whole blood at 37°C for 1 hr and stained with PE-Cy5.5 anti-rabbit IgG antibody. By flow cytometry, attachment and phagocytic processes could be detected, simultaneously. The established method is a valuable tool for diagnosis of phagocytic disorder and study of molecules involved in phagocytosis. 2017-09-28T04:21:53Z 2017-09-28T04:21:53Z 2016-09-02 Journal 15321819 2-s2.0-84975512237 10.1080/15321819.2016.1171780 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84975512237&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/41547
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016 Taylor & Francis. The current available assays cannot differentiate the stages of phagocytosis. We, therefore, established methods for concurrent detection of antigen attachment and engulfment by phagocyte using latex beads coated with lipopolysaccharide, rabbit IgG, and carboxyfluorescein diacetate succinimidyl ester. The generated beads were incubated with whole blood at 37°C for 1 hr and stained with PE-Cy5.5 anti-rabbit IgG antibody. By flow cytometry, attachment and phagocytic processes could be detected, simultaneously. The established method is a valuable tool for diagnosis of phagocytic disorder and study of molecules involved in phagocytosis.
format Journal
author Laopajon W.
Takheaw N.
Kasinrerk W.
Pata S.
spellingShingle Laopajon W.
Takheaw N.
Kasinrerk W.
Pata S.
Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
author_facet Laopajon W.
Takheaw N.
Kasinrerk W.
Pata S.
author_sort Laopajon W.
title Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
title_short Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
title_full Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
title_fullStr Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
title_full_unstemmed Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
title_sort simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84975512237&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41547
_version_ 1681422022137085952

Similar Items