Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability deter...

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Main Authors:	Intorasoot S., Techateerawat J., Intorasoot A.
Format:	Article
Language:	English
Published:	2014
Online Access:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84880650274&partnerID=40&md5=3e70c0a36752093afcee52cfe053a94a http://cmuir.cmu.ac.th/handle/6653943832/4162
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Institution:	Chiang Mai University
Language:	English

id	th-cmuir.6653943832-4162
record_format	dspace
spelling	th-cmuir.6653943832-41622014-08-30T02:35:44Z Genomic DNA isolation from dried blood using gelatin-coated magnetic particles Intorasoot S. Techateerawat J. Intorasoot A. Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silicabased kit. In addition, the quality of extracted DNA was determined using β-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications. 2014-08-30T02:35:44Z 2014-08-30T02:35:44Z 2013 Article 00113891 CUSCA http://www.scopus.com/inward/record.url?eid=2-s2.0-84880650274&partnerID=40&md5=3e70c0a36752093afcee52cfe053a94a http://cmuir.cmu.ac.th/handle/6653943832/4162 English
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
language	English
description	Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silicabased kit. In addition, the quality of extracted DNA was determined using β-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications.
format	Article
author	Intorasoot S. Techateerawat J. Intorasoot A.
spellingShingle	Intorasoot S. Techateerawat J. Intorasoot A. Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
author_facet	Intorasoot S. Techateerawat J. Intorasoot A.
author_sort	Intorasoot S.
title	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_short	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_full	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_fullStr	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_full_unstemmed	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_sort	genomic dna isolation from dried blood using gelatin-coated magnetic particles
publishDate	2014
url	http://www.scopus.com/inward/record.url?eid=2-s2.0-84880650274&partnerID=40&md5=3e70c0a36752093afcee52cfe053a94a http://cmuir.cmu.ac.th/handle/6653943832/4162
_version_	1681420185439830016

Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

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