Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus

Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus

© 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligo...

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Main Authors: Beck I., Payant R., Ngo-Giang-Huong N., Khamduang W., Laomanit L., Jourdain G., Frenkel L.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962283790&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41780
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-417802017-09-28T04:23:19Z Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus Beck I. Payant R. Ngo-Giang-Huong N. Khamduang W. Laomanit L. Jourdain G. Frenkel L. © 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (. OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings. 2017-09-28T04:23:19Z 2017-09-28T04:23:19Z 2016-07-01 Journal 01660934 2-s2.0-84962283790 10.1016/j.jviromet.2016.03.014 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962283790&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/41780
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (. OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings.
format Journal
author Beck I.
Payant R.
Ngo-Giang-Huong N.
Khamduang W.
Laomanit L.
Jourdain G.
Frenkel L.
spellingShingle Beck I.
Payant R.
Ngo-Giang-Huong N.
Khamduang W.
Laomanit L.
Jourdain G.
Frenkel L.
Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
author_facet Beck I.
Payant R.
Ngo-Giang-Huong N.
Khamduang W.
Laomanit L.
Jourdain G.
Frenkel L.
author_sort Beck I.
title Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_short Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_full Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_fullStr Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_full_unstemmed Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_sort development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis b virus
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962283790&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41780
_version_ 1681422065541840896

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