Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions

© 2016 Rithidech, Jangiam, Tungjai, Gordon, Honikel and Whorton. Although the lung is one of the target organs at risk for cancer induction from exposure to heavy ions found in space, information is insufficient on cellular/molecular responses linked to increased cancer risk. Knowledge of such event...

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Main Authors: Rithidech K., Jangiam W., Tungjai M., Gordon C., Honikel L., Whorton E.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84982815683&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41788
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-417882017-09-28T04:23:26Z Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions Rithidech K. Jangiam W. Tungjai M. Gordon C. Honikel L. Whorton E. © 2016 Rithidech, Jangiam, Tungjai, Gordon, Honikel and Whorton. Although the lung is one of the target organs at risk for cancer induction from exposure to heavy ions found in space, information is insufficient on cellular/molecular responses linked to increased cancer risk. Knowledge of such events may aid in the development of new preventive measures. Furthermore, although it is known that germinal cells are sensitive to X- or γ-rays, there is little information on the effects of heavy ions on germinal cells. Our goal was to investigate in vivo effects of 1 GeV/n 48 Ti ions (one of the important heavy ions found in the space environment) on somatic (lung) and germinal (testis) tissues collected at various times after a whole body irradiation of CBA/CaJ mice (0, 0.1, 0.25, or 0.5 Gy, delivered at 1 cGy/min). We hypothesized that 48 Ti-ion-exposure induced damage in both tissues. Lung tissue was collected from each mouse from each treatment group at 1 week, 1 month, and 6 months postirradiation. For the testis, we collected samples at 6 months postirradiation. Hence, only late-occurring effects of 48 Ti ions in the testis were studied. There were five mice per treatment group at each harvest time. We investigated inflammatory responses after exposure to 48 Ti ions by measuring the levels of activated nuclear factor kappa B and selected pro-inflammatory cytokines in both tissues of the same mouse. These measurements were coupled with the quantitation of the levels of global 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Our data clearly showed the induction of chronic inflammation in both tissues of exposed mice. A dose-dependent reduction in global 5hmC was found in the lung at all time-points and in testes collected at 6 months postirradiation. In contrast, significant increases in global 5mC were found only in lung and testes collected at 6 months postirradiation from mice exposed to 0.5 Gy of 1 GeV/n 48 Ti ions. Overall, our data showed that 48 Ti ions may create health risks in both lung and testicular tissues. 2017-09-28T04:23:26Z 2017-09-28T04:23:26Z 2016-06-27 Journal 2-s2.0-84982815683 10.3389/fonc.2016.00155 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84982815683&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/41788
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016 Rithidech, Jangiam, Tungjai, Gordon, Honikel and Whorton. Although the lung is one of the target organs at risk for cancer induction from exposure to heavy ions found in space, information is insufficient on cellular/molecular responses linked to increased cancer risk. Knowledge of such events may aid in the development of new preventive measures. Furthermore, although it is known that germinal cells are sensitive to X- or γ-rays, there is little information on the effects of heavy ions on germinal cells. Our goal was to investigate in vivo effects of 1 GeV/n 48 Ti ions (one of the important heavy ions found in the space environment) on somatic (lung) and germinal (testis) tissues collected at various times after a whole body irradiation of CBA/CaJ mice (0, 0.1, 0.25, or 0.5 Gy, delivered at 1 cGy/min). We hypothesized that 48 Ti-ion-exposure induced damage in both tissues. Lung tissue was collected from each mouse from each treatment group at 1 week, 1 month, and 6 months postirradiation. For the testis, we collected samples at 6 months postirradiation. Hence, only late-occurring effects of 48 Ti ions in the testis were studied. There were five mice per treatment group at each harvest time. We investigated inflammatory responses after exposure to 48 Ti ions by measuring the levels of activated nuclear factor kappa B and selected pro-inflammatory cytokines in both tissues of the same mouse. These measurements were coupled with the quantitation of the levels of global 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Our data clearly showed the induction of chronic inflammation in both tissues of exposed mice. A dose-dependent reduction in global 5hmC was found in the lung at all time-points and in testes collected at 6 months postirradiation. In contrast, significant increases in global 5mC were found only in lung and testes collected at 6 months postirradiation from mice exposed to 0.5 Gy of 1 GeV/n 48 Ti ions. Overall, our data showed that 48 Ti ions may create health risks in both lung and testicular tissues.
format Journal
author Rithidech K.
Jangiam W.
Tungjai M.
Gordon C.
Honikel L.
Whorton E.
spellingShingle Rithidech K.
Jangiam W.
Tungjai M.
Gordon C.
Honikel L.
Whorton E.
Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions
author_facet Rithidech K.
Jangiam W.
Tungjai M.
Gordon C.
Honikel L.
Whorton E.
author_sort Rithidech K.
title Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions
title_short Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions
title_full Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions
title_fullStr Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions
title_full_unstemmed Induction of chronic inflammation and altered levels of DNA hydroxymethylation in somatic and germinal tissues of CBA/CaJ mice exposed to <sup>48</sup>Ti ions
title_sort induction of chronic inflammation and altered levels of dna hydroxymethylation in somatic and germinal tissues of cba/caj mice exposed to <sup>48</sup>ti ions
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84982815683&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41788
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