Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races

Sixteen isoline colonies of Anopheles paraliae were established from wild-caught females collected from cow-baited traps at 4 locations in Thailand. They showed 3 types of X (X1, X2, X3) and 5 types of Y (Y1, Y2, Y3, Y4, Y5) chromosomes based on the number and amount of major block(s) of heterochrom...

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Main Authors: Taai K., Baimai V., Thongsahuan S., Saeung A., Otsuka Y., Srisuka W., Sriwichai P., Somboon P., Jariyapan N., Choochote W.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/23959489
http://cmuir.cmu.ac.th/handle/6653943832/4183
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-41832014-08-30T02:35:46Z Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races Taai K. Baimai V. Thongsahuan S. Saeung A. Otsuka Y. Srisuka W. Sriwichai P. Somboon P. Jariyapan N. Choochote W. Sixteen isoline colonies of Anopheles paraliae were established from wild-caught females collected from cow-baited traps at 4 locations in Thailand. They showed 3 types of X (X1, X2, X3) and 5 types of Y (Y1, Y2, Y3, Y4, Y5) chromosomes based on the number and amount of major block(s) of heterochromatin present in the heterochromatic arm, and were designated as Forms A (X3, Y1), B (X1, X2, X3, Y2), C (X3, Y3), D (X1, X2, X3, Y4) and E (X3, Y5). Form A was found in Songkhla Province, Form B was obtained in Ratchaburi, Nakhon Si Thammarat and Songkhla Provinces, Form C was acquired in Chanthaburi Province, Form D was recovered in Ratchaburi and Songkhla Provinces, and Form E was encountered in Ratchaburi Province. Hybridization experiments among the 7 isoline colonies, which represented the 5 karyotypic forms of An. paraliae, revealed genetic compatibility in providing viable progenies and synaptic salivary gland polytene chromosomes through F2-generations, and thus suggest the conspecific nature of these karyotypic forms. These results were supported by the very low intraspecific sequence divergence (mean genetic distance = 0.000-0.002) of the nucleotide sequences in ribosomal DNA (ITS2) and mitochondrial DNA (COI and COII) of the 5 forms. 2014-08-30T02:35:46Z 2014-08-30T02:35:46Z 2013 Journal Article 0127-5720 23959489 http://www.ncbi.nlm.nih.gov/pubmed/23959489 http://cmuir.cmu.ac.th/handle/6653943832/4183 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Sixteen isoline colonies of Anopheles paraliae were established from wild-caught females collected from cow-baited traps at 4 locations in Thailand. They showed 3 types of X (X1, X2, X3) and 5 types of Y (Y1, Y2, Y3, Y4, Y5) chromosomes based on the number and amount of major block(s) of heterochromatin present in the heterochromatic arm, and were designated as Forms A (X3, Y1), B (X1, X2, X3, Y2), C (X3, Y3), D (X1, X2, X3, Y4) and E (X3, Y5). Form A was found in Songkhla Province, Form B was obtained in Ratchaburi, Nakhon Si Thammarat and Songkhla Provinces, Form C was acquired in Chanthaburi Province, Form D was recovered in Ratchaburi and Songkhla Provinces, and Form E was encountered in Ratchaburi Province. Hybridization experiments among the 7 isoline colonies, which represented the 5 karyotypic forms of An. paraliae, revealed genetic compatibility in providing viable progenies and synaptic salivary gland polytene chromosomes through F2-generations, and thus suggest the conspecific nature of these karyotypic forms. These results were supported by the very low intraspecific sequence divergence (mean genetic distance = 0.000-0.002) of the nucleotide sequences in ribosomal DNA (ITS2) and mitochondrial DNA (COI and COII) of the 5 forms.
format Article
author Taai K.
Baimai V.
Thongsahuan S.
Saeung A.
Otsuka Y.
Srisuka W.
Sriwichai P.
Somboon P.
Jariyapan N.
Choochote W.
spellingShingle Taai K.
Baimai V.
Thongsahuan S.
Saeung A.
Otsuka Y.
Srisuka W.
Sriwichai P.
Somboon P.
Jariyapan N.
Choochote W.
Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races
author_facet Taai K.
Baimai V.
Thongsahuan S.
Saeung A.
Otsuka Y.
Srisuka W.
Sriwichai P.
Somboon P.
Jariyapan N.
Choochote W.
author_sort Taai K.
title Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races
title_short Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races
title_full Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races
title_fullStr Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races
title_full_unstemmed Metaphase karyotypes of Anopheles paraliae (Diptera:Culicidae) in Thailand and evidence to support five cytological races
title_sort metaphase karyotypes of anopheles paraliae (diptera:culicidae) in thailand and evidence to support five cytological races
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/23959489
http://cmuir.cmu.ac.th/handle/6653943832/4183
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