Multiparameter optimization method and enhanced production of secreted recombinant single-chain variable fragment against the HIV-1 P17 protein from Escherichia coli by fed-batch fermentation

• Main Authors: Paopang P., Kasinrerk W., Tayapiwatana C., Seesuriyachan P., Butr-Indr B.
• Format: Journal
• Published: 2017
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84968645187&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/41960
• Institution: Chiang Mai University

© 2016, Taylor & Francis Group, LLC. The single-chain fragment variable (scFv) was used to produce a completely functional antigen-binding fragment in bacterial systems. The advancements in antibody engineering have simplified the method of producing Fv fragments and made it more efficient and...