Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil

• Main Authors: Duangmal K., Suksaard P., Pathom-Aree W., Mingma R., Matsumoto A., Takahashi Y.
• Format: Journal
• Published: 2017
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84964308937&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/41988
• Institution: Chiang Mai University

© 2016 IUMS. The taxonomic position of the halophilic actinobacterial strain, HS05-03 T , isolated from solar saltern soil, was determined using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence of the strain showed that it formed a distinct evolutionary lineage in the genus Actinopolyspora. The organism was most closely related to the type strains of the species Actinopolyspora xinjiangensis (98.0 % similarity), Actinopolyspora righensis (97.9 % similarity), Actinopolyspora lacussalsi (97.9 % similarity) and Actinopolyspora erythraea (97.8 % similarity). The whole-organism hydrolysates contained meso-diaminopimelic acid, arabinose, galactose and ribose. The predominant menaquinones were found to be MK-9(H 4 ) and MK-10(H 4 ). The acyl type of the peptidoglycan was N-acetyl. The diagnostic phospholipid detected was phosphatidylcholine. The predominant cellular fatty acids were iso-C 16: 0 , anteiso-C 17 : 0 and iso-C 15: 0 . The G+C content of the genomic DNA was 69.9 mol%. DNA-DNA hybridization values between strain HS05-03T and the type strains of the most closely related species were below the 70 % threshold. On the basis of the phenotypic and genotypic data, it is proposed that strain HS05-03 T represents a novel species of the genus Actinopolyspora, with the name Actinopolyspora salinaria sp. nov. The type strain is HS05-03 T (=BCC 51286 T =NBRC 109078 T ).