Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei

Penicillium marneffei is a significant opportunistic fungal pathogen in Southeast Asia and its ability to survive inside the host macrophages is believed to be important in the establishment of infection. Previously, we isolated a gene encoding a catalase- peroxidase (cpeA) from P. marneffei and sho...

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Main Authors: Pongpom M., Sawatdeechaikul P., Kummasook A., Khanthawong S., Vanittanakom N.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84885907409&partnerID=40&md5=560cbaf289b9c6fbf5c5a3452fccdd0a
http://cmuir.cmu.ac.th/handle/6653943832/4200
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spelling th-cmuir.6653943832-42002014-08-30T02:35:47Z Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei Pongpom M. Sawatdeechaikul P. Kummasook A. Khanthawong S. Vanittanakom N. Penicillium marneffei is a significant opportunistic fungal pathogen in Southeast Asia and its ability to survive inside the host macrophages is believed to be important in the establishment of infection. Previously, we isolated a gene encoding a catalase- peroxidase (cpeA) from P. marneffei and showed that the cpeA transcript is specifically upregulated during yeast phase growth at 37°C. In this study, the cpeA transcript was found to be induced during the mycelium to yeast phase transition and during stress conditions induced by hydrogen peroxide treatment. Null mutation of cpeA reduced the fungal tolerance to hydrogen peroxide but not to heat stress. These results indicated that the CpeA plays a crucial role in this fungus' oxidative stress response. Western blot analysis demonstrated that the CpeA induced antibody production in P. marneffei-infected patients, including highly exposed-healthy people. This is the first report that the catalase-peroxidase possesses an immunogenic property in fungi. © 2013 ISHAM. 2014-08-30T02:35:47Z 2014-08-30T02:35:47Z 2013 Article 13693786 10.3109/13693786.2013.807445 MEMYF http://www.scopus.com/inward/record.url?eid=2-s2.0-84885907409&partnerID=40&md5=560cbaf289b9c6fbf5c5a3452fccdd0a http://cmuir.cmu.ac.th/handle/6653943832/4200 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Penicillium marneffei is a significant opportunistic fungal pathogen in Southeast Asia and its ability to survive inside the host macrophages is believed to be important in the establishment of infection. Previously, we isolated a gene encoding a catalase- peroxidase (cpeA) from P. marneffei and showed that the cpeA transcript is specifically upregulated during yeast phase growth at 37°C. In this study, the cpeA transcript was found to be induced during the mycelium to yeast phase transition and during stress conditions induced by hydrogen peroxide treatment. Null mutation of cpeA reduced the fungal tolerance to hydrogen peroxide but not to heat stress. These results indicated that the CpeA plays a crucial role in this fungus' oxidative stress response. Western blot analysis demonstrated that the CpeA induced antibody production in P. marneffei-infected patients, including highly exposed-healthy people. This is the first report that the catalase-peroxidase possesses an immunogenic property in fungi. © 2013 ISHAM.
format Article
author Pongpom M.
Sawatdeechaikul P.
Kummasook A.
Khanthawong S.
Vanittanakom N.
spellingShingle Pongpom M.
Sawatdeechaikul P.
Kummasook A.
Khanthawong S.
Vanittanakom N.
Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
author_facet Pongpom M.
Sawatdeechaikul P.
Kummasook A.
Khanthawong S.
Vanittanakom N.
author_sort Pongpom M.
title Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_short Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_full Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_fullStr Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_full_unstemmed Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_sort antioxidative and immunogenic properties of catalase-peroxidase protein in penicillium marneffei
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84885907409&partnerID=40&md5=560cbaf289b9c6fbf5c5a3452fccdd0a
http://cmuir.cmu.ac.th/handle/6653943832/4200
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