Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs

© 2016 Heart Rhythm Society. All rights reserved. Background The effects of intermittent open-loop vagal nerve stimulation (VNS) on the ventricular rate (VR) during atrial fibrillation (AF) remain unclear. Objective The purpose of this study was to test the hypothesis that VNS damages the stellate g...

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Main Authors: Chinda K., Tsai W., Chan Y., Lin A., Patel J., Zhao Y., Tan A., Shen M., Lin H., Shen C., Chattipakorn N., Rubart-Von Der Lohe M., Chen L., Fishbein M., Lin S., Chen Z., Chen P.
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Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958890232&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42071
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spelling th-cmuir.6653943832-420712017-09-28T04:25:05Z Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs Chinda K. Tsai W. Chan Y. Lin A. Patel J. Zhao Y. Tan A. Shen M. Lin H. Shen C. Chattipakorn N. Rubart-Von Der Lohe M. Chen L. Fishbein M. Lin S. Chen Z. Chen P. © 2016 Heart Rhythm Society. All rights reserved. Background The effects of intermittent open-loop vagal nerve stimulation (VNS) on the ventricular rate (VR) during atrial fibrillation (AF) remain unclear. Objective The purpose of this study was to test the hypothesis that VNS damages the stellate ganglion (SG) and improves VR control during persistent AF. Methods We performed left cervical VNS in ambulatory dogs while recording the left SG nerve activity (SGNA) and vagal nerve activity. Tyrosine hydroxylase (TH) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to assess neuronal cell death in the SG. Results We induced persistent AF by atrial pacing in 6 dogs, followed by intermittent VNS with short ON-time (14 seconds) and long OFF-time (66 seconds). The integrated SGNA and VR during AF were 4.84 mV·s (95% confidence interval [CI] 3.08-6.60 mV·s) and 142 beats/min (95% CI 116-168 beats/min), respectively. During AF, VNS reduced the integrated SGNA and VR, respectively, to 3.74 mV·s (95% CI 2.27-5.20 mV·s; P =.021) and 115 beats/min (95% CI 96-134 beats/min; P =.016) during 66-second OFF-time and to 4.07 mV·s (95% CI 2.42-5.72 mV·s; P =.037) and 114 beats/min (95% CI 83-146 beats/min; P =.039) during 3-minute OFF-time. VNS increased the frequencies of prolonged ( > 3 seconds) pauses during AF. TH staining showed large confluent areas of damage in the left SG, characterized by pyknotic nuclei, reduced TH staining, increased percentage of TH-negative ganglion cells, and positive TUNEL staining. Occasional TUNEL-positive ganglion cells were also observed in the right SG. Conclusion VNS damaged the SG, leading to reduced SGNA and better rate control during persistent AF. 2017-09-28T04:25:05Z 2017-09-28T04:25:05Z 2016-03-01 Journal 15475271 2-s2.0-84958890232 10.1016/j.hrthm.2015.11.031 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958890232&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/42071
institution Chiang Mai University
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description © 2016 Heart Rhythm Society. All rights reserved. Background The effects of intermittent open-loop vagal nerve stimulation (VNS) on the ventricular rate (VR) during atrial fibrillation (AF) remain unclear. Objective The purpose of this study was to test the hypothesis that VNS damages the stellate ganglion (SG) and improves VR control during persistent AF. Methods We performed left cervical VNS in ambulatory dogs while recording the left SG nerve activity (SGNA) and vagal nerve activity. Tyrosine hydroxylase (TH) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to assess neuronal cell death in the SG. Results We induced persistent AF by atrial pacing in 6 dogs, followed by intermittent VNS with short ON-time (14 seconds) and long OFF-time (66 seconds). The integrated SGNA and VR during AF were 4.84 mV·s (95% confidence interval [CI] 3.08-6.60 mV·s) and 142 beats/min (95% CI 116-168 beats/min), respectively. During AF, VNS reduced the integrated SGNA and VR, respectively, to 3.74 mV·s (95% CI 2.27-5.20 mV·s; P =.021) and 115 beats/min (95% CI 96-134 beats/min; P =.016) during 66-second OFF-time and to 4.07 mV·s (95% CI 2.42-5.72 mV·s; P =.037) and 114 beats/min (95% CI 83-146 beats/min; P =.039) during 3-minute OFF-time. VNS increased the frequencies of prolonged ( > 3 seconds) pauses during AF. TH staining showed large confluent areas of damage in the left SG, characterized by pyknotic nuclei, reduced TH staining, increased percentage of TH-negative ganglion cells, and positive TUNEL staining. Occasional TUNEL-positive ganglion cells were also observed in the right SG. Conclusion VNS damaged the SG, leading to reduced SGNA and better rate control during persistent AF.
format Journal
author Chinda K.
Tsai W.
Chan Y.
Lin A.
Patel J.
Zhao Y.
Tan A.
Shen M.
Lin H.
Shen C.
Chattipakorn N.
Rubart-Von Der Lohe M.
Chen L.
Fishbein M.
Lin S.
Chen Z.
Chen P.
spellingShingle Chinda K.
Tsai W.
Chan Y.
Lin A.
Patel J.
Zhao Y.
Tan A.
Shen M.
Lin H.
Shen C.
Chattipakorn N.
Rubart-Von Der Lohe M.
Chen L.
Fishbein M.
Lin S.
Chen Z.
Chen P.
Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
author_facet Chinda K.
Tsai W.
Chan Y.
Lin A.
Patel J.
Zhao Y.
Tan A.
Shen M.
Lin H.
Shen C.
Chattipakorn N.
Rubart-Von Der Lohe M.
Chen L.
Fishbein M.
Lin S.
Chen Z.
Chen P.
author_sort Chinda K.
title Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
title_short Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
title_full Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
title_fullStr Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
title_full_unstemmed Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
title_sort intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958890232&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42071
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