HPV genotyping in adenocarcinoma of the uterine cervix in Thailand

Objective To determine the distribution of human papillomavirus (HPV) genotypes in cervical adenocarcinoma in Thailand and to evaluate the clinicopathologic characteristics associated with common HPV genotypes. Methods Formalin-fixed, paraffin-embedded tissues from 150 patients with adenocarcinoma w...

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Main Authors: Siriaunkgul S., Utaipat U., Suthipintawong C., Tungsinmunkong K., Triratanachat S., Khunamornpong S.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84887101288&partnerID=40&md5=86b64f3c0170b90d94e406ad14e769d2
http://cmuir.cmu.ac.th/handle/6653943832/4209
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-42092014-08-30T02:35:47Z HPV genotyping in adenocarcinoma of the uterine cervix in Thailand Siriaunkgul S. Utaipat U. Suthipintawong C. Tungsinmunkong K. Triratanachat S. Khunamornpong S. Objective To determine the distribution of human papillomavirus (HPV) genotypes in cervical adenocarcinoma in Thailand and to evaluate the clinicopathologic characteristics associated with common HPV genotypes. Methods Formalin-fixed, paraffin-embedded tissues from 150 patients with adenocarcinoma were collected from 4 areas of Thailand. Infection with HPV was detected by nested polymerase chain reaction (PCR) with primers MY09/11 and GP5 +/6 +. Genotyping was performed using a linear array assay, followed by type-specific PCR targeting the E6/E7 regions of HPV-16, HPV-18, and HPV-52 if the linear array test was negative. Results Human papillomavirus DNA was detected in 145 (97%) adenocarcinomas (132 single infections; 11 multiple infections; 2 tumors with undetermined HPV type). Genotype 18 was most common (66%), followed by HPV-16 (30%) and HPV-45 (3%). Infection with only HPV-16 and/or HPV-18 accounted for 88% of the HPV-positive tumors. Patients with HPV-18 infection had a younger age (P = 0.009) and higher tumor grade (P < 0.001) than patients with HPV-16 infection. Conclusion The HPV detection rate in cervical adenocarcinomas in Thailand is high. The predominant genotype is HPV-18, being twice as common as HPV-16. Genotype variations are associated with patient age and tumor grade. Vaccination against HPV-16/HPV-18 might prevent almost 90% of adenocarcinomas. © 2013 International Federation of Gynecology and Obstetrics. 2014-08-30T02:35:47Z 2014-08-30T02:35:47Z 2013 Article 00207292 10.1016/j.ijgo.2013.06.034 IJGOA http://www.scopus.com/inward/record.url?eid=2-s2.0-84887101288&partnerID=40&md5=86b64f3c0170b90d94e406ad14e769d2 http://cmuir.cmu.ac.th/handle/6653943832/4209 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Objective To determine the distribution of human papillomavirus (HPV) genotypes in cervical adenocarcinoma in Thailand and to evaluate the clinicopathologic characteristics associated with common HPV genotypes. Methods Formalin-fixed, paraffin-embedded tissues from 150 patients with adenocarcinoma were collected from 4 areas of Thailand. Infection with HPV was detected by nested polymerase chain reaction (PCR) with primers MY09/11 and GP5 +/6 +. Genotyping was performed using a linear array assay, followed by type-specific PCR targeting the E6/E7 regions of HPV-16, HPV-18, and HPV-52 if the linear array test was negative. Results Human papillomavirus DNA was detected in 145 (97%) adenocarcinomas (132 single infections; 11 multiple infections; 2 tumors with undetermined HPV type). Genotype 18 was most common (66%), followed by HPV-16 (30%) and HPV-45 (3%). Infection with only HPV-16 and/or HPV-18 accounted for 88% of the HPV-positive tumors. Patients with HPV-18 infection had a younger age (P = 0.009) and higher tumor grade (P < 0.001) than patients with HPV-16 infection. Conclusion The HPV detection rate in cervical adenocarcinomas in Thailand is high. The predominant genotype is HPV-18, being twice as common as HPV-16. Genotype variations are associated with patient age and tumor grade. Vaccination against HPV-16/HPV-18 might prevent almost 90% of adenocarcinomas. © 2013 International Federation of Gynecology and Obstetrics.
format Article
author Siriaunkgul S.
Utaipat U.
Suthipintawong C.
Tungsinmunkong K.
Triratanachat S.
Khunamornpong S.
spellingShingle Siriaunkgul S.
Utaipat U.
Suthipintawong C.
Tungsinmunkong K.
Triratanachat S.
Khunamornpong S.
HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
author_facet Siriaunkgul S.
Utaipat U.
Suthipintawong C.
Tungsinmunkong K.
Triratanachat S.
Khunamornpong S.
author_sort Siriaunkgul S.
title HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_short HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_full HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_fullStr HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_full_unstemmed HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_sort hpv genotyping in adenocarcinoma of the uterine cervix in thailand
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84887101288&partnerID=40&md5=86b64f3c0170b90d94e406ad14e769d2
http://cmuir.cmu.ac.th/handle/6653943832/4209
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