Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan

© 2016 Wiley Periodicals, Inc. This study investigated the genetic variability of astroviruses in children with acute gastroenteritis in Japan in 2012 to 2013. A total of 330 fecal specimens collected from children with diarrhea were tested for astroviruses using RT-PCR and nucleotide sequencing tec...

Full description

Saved in:
Bibliographic Details
Main Authors: Khamrin P., Thongprachum A., Okitsu S., Hayakawa S., Maneekarn N., Ushijima H.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84954388445&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42114
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-42114
record_format dspace
spelling th-cmuir.6653943832-421142017-09-28T04:25:18Z Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan Khamrin P. Thongprachum A. Okitsu S. Hayakawa S. Maneekarn N. Ushijima H. © 2016 Wiley Periodicals, Inc. This study investigated the genetic variability of astroviruses in children with acute gastroenteritis in Japan in 2012 to 2013. A total of 330 fecal specimens collected from children with diarrhea were tested for astroviruses using RT-PCR and nucleotide sequencing techniques. Among the tested specimens, 54 (16.4%) were positive for astrovirus. Three different phylogenetic clades were detected. MLB was the most predominant clade (35 samples), followed by classic astrovirus (17 samples), and VA astrovirus (2 samples). Two types of MLB were identified (MLB1 and MLB2), while classic astroviruses were HAstV1 and HAstV4. Two VA astroviruses were identified as VA2. 2017-09-28T04:25:18Z 2017-09-28T04:25:18Z 2016-02-01 Journal 01466615 2-s2.0-84954388445 10.1002/jmv.24337 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84954388445&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/42114
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016 Wiley Periodicals, Inc. This study investigated the genetic variability of astroviruses in children with acute gastroenteritis in Japan in 2012 to 2013. A total of 330 fecal specimens collected from children with diarrhea were tested for astroviruses using RT-PCR and nucleotide sequencing techniques. Among the tested specimens, 54 (16.4%) were positive for astrovirus. Three different phylogenetic clades were detected. MLB was the most predominant clade (35 samples), followed by classic astrovirus (17 samples), and VA astrovirus (2 samples). Two types of MLB were identified (MLB1 and MLB2), while classic astroviruses were HAstV1 and HAstV4. Two VA astroviruses were identified as VA2.
format Journal
author Khamrin P.
Thongprachum A.
Okitsu S.
Hayakawa S.
Maneekarn N.
Ushijima H.
spellingShingle Khamrin P.
Thongprachum A.
Okitsu S.
Hayakawa S.
Maneekarn N.
Ushijima H.
Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
author_facet Khamrin P.
Thongprachum A.
Okitsu S.
Hayakawa S.
Maneekarn N.
Ushijima H.
author_sort Khamrin P.
title Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
title_short Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
title_full Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
title_fullStr Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
title_full_unstemmed Multiple astrovirus MLB1, MLB2, VA2 clades, and classic human astrovirus in children with acute gastroenteritis in Japan
title_sort multiple astrovirus mlb1, mlb2, va2 clades, and classic human astrovirus in children with acute gastroenteritis in japan
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84954388445&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42114
_version_ 1681422128041164800