Early detection of trichinella spiralis in muscle of infected mice by real-time fluorescence resonance energy transfer PCR

• Main Authors: Tantrawatpan C., Intapan P.M., Thanchomnang T., Sanpool O., Janwan P., Boonmars T., Morakote N., Maleewong W.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-84884223269&partnerID=40&md5=e3d3e04a6a3f05c7450804bf15a8790b; http://cmuir.cmu.ac.th/handle/6653943832/4215
• Institution: Chiang Mai University

Real-time fluorescence resonance energy transfer (FRET) PCR and melting curve analysis using newly developed fluorophore-labeled hybridization probes were applied for the detection of Trichinella spiralis DNA in muscle of mice following oral inoculation with 300 T. spiralis larvae. The developed ass...