Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users

We evaluated a quantitative enzyme immunoassay (trak-C) for hepatitis C virus core antigen (HCV core Ag) by testing serum specimens from 820 injection drug users in Thailand with anti-HCV antibodies. The HCV genotypes in this population include genotypes 3 and 6, which have not been extensively test...

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Main Authors: Netski D.M., Wang X.-H., Mehta S.H., Nelson K., Celentano D., Thongsawat S., Maneekarn N., Suriyanon V., Jittiwutikorn J., Thomas D.L., Ticehurst J.R.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-11844255837&partnerID=40&md5=819049ba37485d39d93d6c3c894f38ce
http://cmuir.cmu.ac.th/handle/6653943832/4223
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spelling th-cmuir.6653943832-42232014-08-30T02:35:48Z Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users Netski D.M. Wang X.-H. Mehta S.H. Nelson K. Celentano D. Thongsawat S. Maneekarn N. Suriyanon V. Jittiwutikorn J. Thomas D.L. Ticehurst J.R. We evaluated a quantitative enzyme immunoassay (trak-C) for hepatitis C virus core antigen (HCV core Ag) by testing serum specimens from 820 injection drug users in Thailand with anti-HCV antibodies. The HCV genotypes in this population include genotypes 3 and 6, which have not been extensively tested with this assay. Among these specimens, 629 (76.7%) yielded positive results, with HCV core Ag concentrations predominantly spanning (35.7%) or above (58.2%) the measurable range of 1.5 to 100 pg/ml. To assess reproducibility, we retested 30 specimens representing six core Ag ranges; the mean coefficient of variation for each range was ≤9.7% (highest for 1.5 to 25 pg/ml). We also tested 204 specimens of the 820-specimen set for HCV RNA: while 146 (71.6%) were core Ag positive, 168 (82.4%) had detectable HCV RNA, of which 96% were typeable as genotype 3 (39%), 1 (31%), or 6 (26%) by nested reverse transcription-PCR. Among RNA-positive specimens, 86.9% had core Ag; 94% of the RNA negatives were core Ag negative. While there was no apparent bias for detecting core Ag representing the tested genotypes, median quantified results were higher for types 1a and 6 than for genotype 3 (P = 0.01); similarly, the median core Ag concentration was higher in HCV-human immunodeficiency virus-coinfected subjects than in HCV-monoinfected subjects. Our results demonstrated a good correlation between core Ag and HCV RNA in this population with high frequencies of genotypes 3 and 6. Because most core Ag concentrations were greater than those in the measurable range, we recommend a 10-fold dilution of the specimen before quantification. Reproducibility, low technical requirements, and high throughput should make this assay useful for clinical or research monitoring of HCV levels during active infection. 2014-08-30T02:35:48Z 2014-08-30T02:35:48Z 2004 Article 00951137 10.1128/JCM.42.4.1631-1636.2004 15071017 JCMID http://www.scopus.com/inward/record.url?eid=2-s2.0-11844255837&partnerID=40&md5=819049ba37485d39d93d6c3c894f38ce http://cmuir.cmu.ac.th/handle/6653943832/4223 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description We evaluated a quantitative enzyme immunoassay (trak-C) for hepatitis C virus core antigen (HCV core Ag) by testing serum specimens from 820 injection drug users in Thailand with anti-HCV antibodies. The HCV genotypes in this population include genotypes 3 and 6, which have not been extensively tested with this assay. Among these specimens, 629 (76.7%) yielded positive results, with HCV core Ag concentrations predominantly spanning (35.7%) or above (58.2%) the measurable range of 1.5 to 100 pg/ml. To assess reproducibility, we retested 30 specimens representing six core Ag ranges; the mean coefficient of variation for each range was ≤9.7% (highest for 1.5 to 25 pg/ml). We also tested 204 specimens of the 820-specimen set for HCV RNA: while 146 (71.6%) were core Ag positive, 168 (82.4%) had detectable HCV RNA, of which 96% were typeable as genotype 3 (39%), 1 (31%), or 6 (26%) by nested reverse transcription-PCR. Among RNA-positive specimens, 86.9% had core Ag; 94% of the RNA negatives were core Ag negative. While there was no apparent bias for detecting core Ag representing the tested genotypes, median quantified results were higher for types 1a and 6 than for genotype 3 (P = 0.01); similarly, the median core Ag concentration was higher in HCV-human immunodeficiency virus-coinfected subjects than in HCV-monoinfected subjects. Our results demonstrated a good correlation between core Ag and HCV RNA in this population with high frequencies of genotypes 3 and 6. Because most core Ag concentrations were greater than those in the measurable range, we recommend a 10-fold dilution of the specimen before quantification. Reproducibility, low technical requirements, and high throughput should make this assay useful for clinical or research monitoring of HCV levels during active infection.
format Article
author Netski D.M.
Wang X.-H.
Mehta S.H.
Nelson K.
Celentano D.
Thongsawat S.
Maneekarn N.
Suriyanon V.
Jittiwutikorn J.
Thomas D.L.
Ticehurst J.R.
spellingShingle Netski D.M.
Wang X.-H.
Mehta S.H.
Nelson K.
Celentano D.
Thongsawat S.
Maneekarn N.
Suriyanon V.
Jittiwutikorn J.
Thomas D.L.
Ticehurst J.R.
Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users
author_facet Netski D.M.
Wang X.-H.
Mehta S.H.
Nelson K.
Celentano D.
Thongsawat S.
Maneekarn N.
Suriyanon V.
Jittiwutikorn J.
Thomas D.L.
Ticehurst J.R.
author_sort Netski D.M.
title Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users
title_short Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users
title_full Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users
title_fullStr Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users
title_full_unstemmed Hepatitis C Virus (HCV) Core Antigen Assay to Detect Ongoing HCV Infection in Thai Injection Drug Users
title_sort hepatitis c virus (hcv) core antigen assay to detect ongoing hcv infection in thai injection drug users
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-11844255837&partnerID=40&md5=819049ba37485d39d93d6c3c894f38ce
http://cmuir.cmu.ac.th/handle/6653943832/4223
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