Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum

• Main Authors: Uparanukraw P., Morakote N., Harnnoi T., Dantrakool A.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-0034896063&partnerID=40&md5=175953ad20343da325b9d2c1f2b8b4db; http://www.ncbi.nlm.nih.gov/pubmed/11570561; http://cmuir.cmu.ac.th/handle/6653943832/4227
• Institution: Chiang Mai University

The advanced third-stage larvae (aL3) of Gnathostoma spinigerum contain a 24 kDa glycoprotein with diagnostic potential. Immunoscreening with the monoclonal antibody to the 24-kDa protein (mAb GN6/24) has identified a cDNA clone with an insert of 932 base pairs (bp). The insert contains a full-length gene of 732 bp encoding a protein that is 33-39% similar to matrix metalloproteinases (MMPs) of Caenorhabditis elegans and several lower and higher vertebrates. The MMP-like protein of G. spinigerum possesses the catalytic domain, but lacks the propeptide and hemopexin-like domains found in other MMPs. A signal peptide of 23 amino acids at its amino terminus indicates that it is a secretory protein, which is confirmed by Western blot analysis showing the presence of the 24 kDa protein in the excretory-secretory products of aL3.