Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells

© 2016 Kewalin Inthanon et al. Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and p...

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Main Authors: Inthanon K., Daranarong D., Techaikool P., Punyodom W., Khaniyao V., Bernstein A., Wongkham W.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84956888654&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42434
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-424342017-09-28T04:27:05Z Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells Inthanon K. Daranarong D. Techaikool P. Punyodom W. Khaniyao V. Bernstein A. Wongkham W. © 2016 Kewalin Inthanon et al. Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a noncytotoxic release profile. The hydrophilic properties of the membranes were 35-40% higher than those of a standard PLCL and commercial polystyrene (PS). The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N-H, and C-N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes. These membranes enhanced human Wharton's jelly mesenchymal stem cells (hWJMSC) proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK). On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin. These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications. 2017-09-28T04:27:05Z 2017-09-28T04:27:05Z 2016-01-01 Journal 2-s2.0-84956888654 10.1155/2016/5309484 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84956888654&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/42434
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016 Kewalin Inthanon et al. Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a noncytotoxic release profile. The hydrophilic properties of the membranes were 35-40% higher than those of a standard PLCL and commercial polystyrene (PS). The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N-H, and C-N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes. These membranes enhanced human Wharton's jelly mesenchymal stem cells (hWJMSC) proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK). On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin. These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications.
format Journal
author Inthanon K.
Daranarong D.
Techaikool P.
Punyodom W.
Khaniyao V.
Bernstein A.
Wongkham W.
spellingShingle Inthanon K.
Daranarong D.
Techaikool P.
Punyodom W.
Khaniyao V.
Bernstein A.
Wongkham W.
Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells
author_facet Inthanon K.
Daranarong D.
Techaikool P.
Punyodom W.
Khaniyao V.
Bernstein A.
Wongkham W.
author_sort Inthanon K.
title Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells
title_short Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells
title_full Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells
title_fullStr Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells
title_full_unstemmed Biocompatibility assessment of PLCL-sericin copolymer membranes using Wharton's jelly mesenchymal stem cells
title_sort biocompatibility assessment of plcl-sericin copolymer membranes using wharton's jelly mesenchymal stem cells
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84956888654&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42434
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