Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1

© 2016 Arunee Thanasarasakulpong et al. Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immun...

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Main Authors: Thanasarasakulpong A., Poolperm P., Tangjitjaroen W., Varinrak T., Sawada T., Pfeiffer D., Sthitmatee N.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958581641&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42517
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spelling th-cmuir.6653943832-425172017-09-28T04:27:33Z Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1 Thanasarasakulpong A. Poolperm P. Tangjitjaroen W. Varinrak T. Sawada T. Pfeiffer D. Sthitmatee N. © 2016 Arunee Thanasarasakulpong et al. Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immunogenicity of rOmpH purified using electroelution with rOmpH purified using affinity chromatography and native OmpH purified using electroelution and a nonimmunized control group. Chickens immunized with rOmpH purified using electroelution produced the highest ELISA antibody levels against P. multocida strains. Chickens in each of the 5 treatment groups were split into two subgroups for challenge with two different P. multocida strains. The average number of adhesions to CEF cells was statistically significantly lower in sera from chickens immunized with rOmpH or native OmpH purified using electroelution than in those of the three other treatment groups. The survival amongst chickens immunized with rOmpH or native OmpH purified using electroelution indicated high levels of protection. In contrast, survival probability was zero or low in the groups immunized with rOmpH purified using affinity chromatography and in the nonimmunized group. These findings show that the rOmpH purified using electroelution retains its immunogenicity and stimulates high levels of protection in chickens against P. multocida infection. 2017-09-28T04:27:33Z 2017-09-28T04:27:33Z 2016-01-01 Journal 2-s2.0-84958581641 10.1155/2016/2579345 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958581641&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/42517
institution Chiang Mai University
building Chiang Mai University Library
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description © 2016 Arunee Thanasarasakulpong et al. Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immunogenicity of rOmpH purified using electroelution with rOmpH purified using affinity chromatography and native OmpH purified using electroelution and a nonimmunized control group. Chickens immunized with rOmpH purified using electroelution produced the highest ELISA antibody levels against P. multocida strains. Chickens in each of the 5 treatment groups were split into two subgroups for challenge with two different P. multocida strains. The average number of adhesions to CEF cells was statistically significantly lower in sera from chickens immunized with rOmpH or native OmpH purified using electroelution than in those of the three other treatment groups. The survival amongst chickens immunized with rOmpH or native OmpH purified using electroelution indicated high levels of protection. In contrast, survival probability was zero or low in the groups immunized with rOmpH purified using affinity chromatography and in the nonimmunized group. These findings show that the rOmpH purified using electroelution retains its immunogenicity and stimulates high levels of protection in chickens against P. multocida infection.
format Journal
author Thanasarasakulpong A.
Poolperm P.
Tangjitjaroen W.
Varinrak T.
Sawada T.
Pfeiffer D.
Sthitmatee N.
spellingShingle Thanasarasakulpong A.
Poolperm P.
Tangjitjaroen W.
Varinrak T.
Sawada T.
Pfeiffer D.
Sthitmatee N.
Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
author_facet Thanasarasakulpong A.
Poolperm P.
Tangjitjaroen W.
Varinrak T.
Sawada T.
Pfeiffer D.
Sthitmatee N.
author_sort Thanasarasakulpong A.
title Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_short Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_full Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_fullStr Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_full_unstemmed Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of pasteurella multocida serovar A:1
title_sort comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein h of pasteurella multocida serovar a:1
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958581641&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42517
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