Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation

© 2016, Czech Academy of Agricultural Sciences. All rights reserved. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curve analy...

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Main Authors: Zambounis A., Samaras A., Xanthopoulou A., Osathanunkul M., Schena L., Tsaftaris A., Madesis P.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84977138855&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42590
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spelling th-cmuir.6653943832-425902017-09-28T04:27:59Z Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation Zambounis A. Samaras A. Xanthopoulou A. Osathanunkul M. Schena L. Tsaftaris A. Madesis P. © 2016, Czech Academy of Agricultural Sciences. All rights reserved. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curve analysis of the amplicons specifically grouped all species into 10 respective unique and distinct HRM curve profiles. The analysis of the normalised HRM melting curves, assigning P. nicotianae as a normalised reference genotype, revealed that the genotype similarities among all the species were adequately low, indicating that Ypt1 marker was sufficient to identify and differentiate the tested species. This HRM method is rapid and reproducible allowing the identification of Phytophthora species and the screening of eventual variants eliminating the separate steps and reducing the risk of contamination. 2017-09-28T04:27:59Z 2017-09-28T04:27:59Z 2016-01-01 Journal 12122580 2-s2.0-84977138855 10.17221/179/2015-PPS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84977138855&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/42590
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2016, Czech Academy of Agricultural Sciences. All rights reserved. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curve analysis of the amplicons specifically grouped all species into 10 respective unique and distinct HRM curve profiles. The analysis of the normalised HRM melting curves, assigning P. nicotianae as a normalised reference genotype, revealed that the genotype similarities among all the species were adequately low, indicating that Ypt1 marker was sufficient to identify and differentiate the tested species. This HRM method is rapid and reproducible allowing the identification of Phytophthora species and the screening of eventual variants eliminating the separate steps and reducing the risk of contamination.
format Journal
author Zambounis A.
Samaras A.
Xanthopoulou A.
Osathanunkul M.
Schena L.
Tsaftaris A.
Madesis P.
spellingShingle Zambounis A.
Samaras A.
Xanthopoulou A.
Osathanunkul M.
Schena L.
Tsaftaris A.
Madesis P.
Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
author_facet Zambounis A.
Samaras A.
Xanthopoulou A.
Osathanunkul M.
Schena L.
Tsaftaris A.
Madesis P.
author_sort Zambounis A.
title Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_short Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_full Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_fullStr Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_full_unstemmed Identification of Phytophthora species by a high resolution melting analysis: An innovative tool for rapid differentiation
title_sort identification of phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84977138855&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/42590
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