Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal composition...

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Main Authors: Biegler B., Yan S., Ortega S., Tennakoon D., Racke M., Karandikar N.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955666022&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43067
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-430672017-09-28T06:47:07Z Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis Biegler B. Yan S. Ortega S. Tennakoon D. Racke M. Karandikar N. Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease. © 2011 Elsevier B.V. 2017-09-28T06:47:06Z 2017-09-28T06:47:06Z 2011-05-01 Journal 01655728 2-s2.0-79955666022 10.1016/j.jneuroim.2011.02.001 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955666022&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43067
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease. © 2011 Elsevier B.V.
format Journal
author Biegler B.
Yan S.
Ortega S.
Tennakoon D.
Racke M.
Karandikar N.
spellingShingle Biegler B.
Yan S.
Ortega S.
Tennakoon D.
Racke M.
Karandikar N.
Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis
author_facet Biegler B.
Yan S.
Ortega S.
Tennakoon D.
Racke M.
Karandikar N.
author_sort Biegler B.
title Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis
title_short Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis
title_full Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis
title_fullStr Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis
title_full_unstemmed Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis
title_sort clonal composition of neuroantigen-specific cd8+ and cd4+ t-cells in multiple sclerosis
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955666022&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43067
_version_ 1681422308480122880

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