Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells

Ischemic heart disease (IHD) is a major factor influencing worldwide mortality rates. Furthermore, IHD has become a significant health problem among the Thai population. Stem cell therapy using mesenchymal stem cells (MSCs) is an alternative therapeutic method that has been applied to improve the qu...

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Main Authors: Runchana Markmee, Sirinda Aungsuchawan, Suteera Narakornsak, Waleephan Tancharoen, Kanokkarn Bumrungkit, Nataporn Pangchaidee, Peraphan Pothacharoen, Chaniporn Puaninta
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/43512
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spelling th-cmuir.6653943832-435122018-04-25T07:36:24Z Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells Runchana Markmee Sirinda Aungsuchawan Suteera Narakornsak Waleephan Tancharoen Kanokkarn Bumrungkit Nataporn Pangchaidee Peraphan Pothacharoen Chaniporn Puaninta Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences Arts and Humanities Ischemic heart disease (IHD) is a major factor influencing worldwide mortality rates. Furthermore, IHD has become a significant health problem among the Thai population. Stem cell therapy using mesenchymal stem cells (MSCs) is an alternative therapeutic method that has been applied to improve the quality of life of patients. Amniotic fluid (AF) contains a heterogeneous cell population, including MSCs, which are multipotent stem cells that have the capability to differentiate into mesenchymal lineages. The purpose of the present study was to evaluate the MSC characteristics of human (h)AF and determine its potency regarding cardiogenic differentiation. MSC characterization following flow cytometric analysis revealed that the cells expressed MSC markers, cluster of differentiation (CD)44, CD90, human leukocyte antigen-ABC and CD73. The results of the alamar blue assay demonstrated that cell proliferation rate continuously increased from the early cultivation phase up to 5-fold during days 1 to 5 of cell culturing. The highest rate of cell proliferation was observed on day 17 with a 30-fold increase compared with that on day 1. During the cardiogenic induction stage, morphological changes were observed between day 0 and day 21, and it was revealed that the hAF derived-MSCs in the cardiogenic-induced group exhibited myotube-like morphology after 7 days of cell culturing. Following cardiogenic induction, immunohistochemistry staining was performed on day 21, and reverse transcription-quantitative polymerase chain reaction on day 7 and 21. These steps were performed to detect the protein and gene expression levels of cardiac specific proteins (GATA4, cardiac troponin T, Nkx2.5 and Connexin43). The results of the present study indicated that hAF-MSCs possess the potential to differentiate into cardiomyocyte-like cells. Thus, it was concluded that hAF may be a suitable source of MSCs for stem cell therapy and tissue engineering. 2018-01-24T03:49:29Z 2018-01-24T03:49:29Z 2017-11-01 Journal 17913004 17912997 2-s2.0-85030124542 10.3892/mmr.2017.7333 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030124542&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43512
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Agricultural and Biological Sciences
Arts and Humanities
spellingShingle Biochemistry, Genetics and Molecular Biology
Agricultural and Biological Sciences
Arts and Humanities
Runchana Markmee
Sirinda Aungsuchawan
Suteera Narakornsak
Waleephan Tancharoen
Kanokkarn Bumrungkit
Nataporn Pangchaidee
Peraphan Pothacharoen
Chaniporn Puaninta
Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells
description Ischemic heart disease (IHD) is a major factor influencing worldwide mortality rates. Furthermore, IHD has become a significant health problem among the Thai population. Stem cell therapy using mesenchymal stem cells (MSCs) is an alternative therapeutic method that has been applied to improve the quality of life of patients. Amniotic fluid (AF) contains a heterogeneous cell population, including MSCs, which are multipotent stem cells that have the capability to differentiate into mesenchymal lineages. The purpose of the present study was to evaluate the MSC characteristics of human (h)AF and determine its potency regarding cardiogenic differentiation. MSC characterization following flow cytometric analysis revealed that the cells expressed MSC markers, cluster of differentiation (CD)44, CD90, human leukocyte antigen-ABC and CD73. The results of the alamar blue assay demonstrated that cell proliferation rate continuously increased from the early cultivation phase up to 5-fold during days 1 to 5 of cell culturing. The highest rate of cell proliferation was observed on day 17 with a 30-fold increase compared with that on day 1. During the cardiogenic induction stage, morphological changes were observed between day 0 and day 21, and it was revealed that the hAF derived-MSCs in the cardiogenic-induced group exhibited myotube-like morphology after 7 days of cell culturing. Following cardiogenic induction, immunohistochemistry staining was performed on day 21, and reverse transcription-quantitative polymerase chain reaction on day 7 and 21. These steps were performed to detect the protein and gene expression levels of cardiac specific proteins (GATA4, cardiac troponin T, Nkx2.5 and Connexin43). The results of the present study indicated that hAF-MSCs possess the potential to differentiate into cardiomyocyte-like cells. Thus, it was concluded that hAF may be a suitable source of MSCs for stem cell therapy and tissue engineering.
format Journal
author Runchana Markmee
Sirinda Aungsuchawan
Suteera Narakornsak
Waleephan Tancharoen
Kanokkarn Bumrungkit
Nataporn Pangchaidee
Peraphan Pothacharoen
Chaniporn Puaninta
author_facet Runchana Markmee
Sirinda Aungsuchawan
Suteera Narakornsak
Waleephan Tancharoen
Kanokkarn Bumrungkit
Nataporn Pangchaidee
Peraphan Pothacharoen
Chaniporn Puaninta
author_sort Runchana Markmee
title Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells
title_short Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells
title_full Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells
title_fullStr Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells
title_full_unstemmed Differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells
title_sort differentiation of mesenchymal stem cells from human amniotic fluid to cardiomyocyte-like cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030124542&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43512
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