A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure

© 2017, Chiang Mai University. All rights reserved. A method for determination of urinary 1-hydroxypyrene (1-OHP) was developed using HPLC-FLD with a column-switching system. 1-OHP in urine samples was extracted by β-glucuronidase hydrolysis, followed by C 18 SPE with optimized conditions. LOD and L...

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Main Authors: Kunrunya Sutan, Warangakana Naksen, Tippawan Prapamontol
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/43596
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spelling th-cmuir.6653943832-435962018-04-25T07:37:35Z A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure Kunrunya Sutan Warangakana Naksen Tippawan Prapamontol Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Agricultural and Biological Sciences Arts and Humanities © 2017, Chiang Mai University. All rights reserved. A method for determination of urinary 1-hydroxypyrene (1-OHP) was developed using HPLC-FLD with a column-switching system. 1-OHP in urine samples was extracted by β-glucuronidase hydrolysis, followed by C 18 SPE with optimized conditions. LOD and LOQ were 0.01 and 0.03 ng/mL, respectively; this was sensitive enough to determine urinary 1-OHP in low-dose exposure. The precision and accuracy of four concentrations (0.06, 2.0, 8.0 and 25 ng/mL) were tested. The intra- and inter-batch precisions were between 1.82-4.22 and 5.01-15.8 %RSD, respectively, while accuracies were between 92.8-117% recoveries, which were in the acceptable range. This method was applied in 130 school children living in a seasonal forest-fire burning area in Northern Thailand. Urinary 1-OHP was detected in all urine samples at concentrations of 1.35±2.26 μmol/mol creatinine, and a range of 0.03-16.0 μmol/mol creatinine. The developed method offers a potential application to determine urinary 1-OHP levels from environmentally low exposure. 2018-01-24T03:50:32Z 2018-01-24T03:50:32Z 2017-10-01 Journal 01252526 2-s2.0-85030658686 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030658686&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43596
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
Arts and Humanities
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
Arts and Humanities
Kunrunya Sutan
Warangakana Naksen
Tippawan Prapamontol
A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure
description © 2017, Chiang Mai University. All rights reserved. A method for determination of urinary 1-hydroxypyrene (1-OHP) was developed using HPLC-FLD with a column-switching system. 1-OHP in urine samples was extracted by β-glucuronidase hydrolysis, followed by C 18 SPE with optimized conditions. LOD and LOQ were 0.01 and 0.03 ng/mL, respectively; this was sensitive enough to determine urinary 1-OHP in low-dose exposure. The precision and accuracy of four concentrations (0.06, 2.0, 8.0 and 25 ng/mL) were tested. The intra- and inter-batch precisions were between 1.82-4.22 and 5.01-15.8 %RSD, respectively, while accuracies were between 92.8-117% recoveries, which were in the acceptable range. This method was applied in 130 school children living in a seasonal forest-fire burning area in Northern Thailand. Urinary 1-OHP was detected in all urine samples at concentrations of 1.35±2.26 μmol/mol creatinine, and a range of 0.03-16.0 μmol/mol creatinine. The developed method offers a potential application to determine urinary 1-OHP levels from environmentally low exposure.
format Journal
author Kunrunya Sutan
Warangakana Naksen
Tippawan Prapamontol
author_facet Kunrunya Sutan
Warangakana Naksen
Tippawan Prapamontol
author_sort Kunrunya Sutan
title A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure
title_short A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure
title_full A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure
title_fullStr A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure
title_full_unstemmed A simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure
title_sort simple high-performance liquid chromatography coupled to fluorescence detection method using column-switching technique for measuring urinary 1-hydroxypyrene from environmental exposure
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030658686&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43596
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