Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract

In the present study, the fractionated extracts of Sesbania grandiflora bark were prepared and evaluated for their biological activities. The ethyl acetate fractionate (EAF) showed high antioxidant activity along with free radical scavenging and reducing mechanisms. The free radical scavenging antio...

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Main Authors: Pimporn Anantaworasakul, Hiroshi Hamamoto, Kazuhisa Sekimizu, Siriporn Okonogi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/43691
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spelling th-cmuir.6653943832-436912018-04-25T07:24:17Z Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract Pimporn Anantaworasakul Hiroshi Hamamoto Kazuhisa Sekimizu Siriporn Okonogi Agricultural and Biological Sciences In the present study, the fractionated extracts of Sesbania grandiflora bark were prepared and evaluated for their biological activities. The ethyl acetate fractionate (EAF) showed high antioxidant activity along with free radical scavenging and reducing mechanisms. The free radical scavenging antioxidant activity of EAF was 69.3 ± 3.6% where its Trolox equivalent antioxidant capacity was 13.6 ± 0.7 mM/mg. EAF exhibited the reducing power equivalent to ferrous sulfate at 152 ± 2 mM/mg and equivalent to gallic acid at 1.05 ± 0.01 mM/mg. In addition, EAF presented high potential on inhibition of bacterial growth with the minimum bactericidal concentration less than 1 mg/mL. Further isolation of EAF using normal-phase open column of silica gel 60, showed that the fractions eluted with the mixture of chloroform and methanol at the ratios of 4:1, 3:2, and 2:3 possessed antibacterial activity. The recovery activity of total different active fractions was 5% EAF, 20 times less than that of EAF. The chromatogram of EAF from a high-performance liquid chromatography was compared with caffeic acid, catechin, coumaric acid, ellagic acid, gallic acid, quercetin, syringin, naringic acid, trans-cinnamic acid, and vanilic acid. The result demonstrated that one major compound of EAF was gallic acid. These results suggest that the fractionated extracts of S. grandiflora bark contained antioxidant and antibacterial activities. 2018-01-24T03:53:44Z 2018-01-24T03:53:44Z 2017-05-30 Journal 18817831 2-s2.0-85032663481 10.5582/ddt.2017.01013 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85032663481&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43691
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Pimporn Anantaworasakul
Hiroshi Hamamoto
Kazuhisa Sekimizu
Siriporn Okonogi
Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract
description In the present study, the fractionated extracts of Sesbania grandiflora bark were prepared and evaluated for their biological activities. The ethyl acetate fractionate (EAF) showed high antioxidant activity along with free radical scavenging and reducing mechanisms. The free radical scavenging antioxidant activity of EAF was 69.3 ± 3.6% where its Trolox equivalent antioxidant capacity was 13.6 ± 0.7 mM/mg. EAF exhibited the reducing power equivalent to ferrous sulfate at 152 ± 2 mM/mg and equivalent to gallic acid at 1.05 ± 0.01 mM/mg. In addition, EAF presented high potential on inhibition of bacterial growth with the minimum bactericidal concentration less than 1 mg/mL. Further isolation of EAF using normal-phase open column of silica gel 60, showed that the fractions eluted with the mixture of chloroform and methanol at the ratios of 4:1, 3:2, and 2:3 possessed antibacterial activity. The recovery activity of total different active fractions was 5% EAF, 20 times less than that of EAF. The chromatogram of EAF from a high-performance liquid chromatography was compared with caffeic acid, catechin, coumaric acid, ellagic acid, gallic acid, quercetin, syringin, naringic acid, trans-cinnamic acid, and vanilic acid. The result demonstrated that one major compound of EAF was gallic acid. These results suggest that the fractionated extracts of S. grandiflora bark contained antioxidant and antibacterial activities.
format Journal
author Pimporn Anantaworasakul
Hiroshi Hamamoto
Kazuhisa Sekimizu
Siriporn Okonogi
author_facet Pimporn Anantaworasakul
Hiroshi Hamamoto
Kazuhisa Sekimizu
Siriporn Okonogi
author_sort Pimporn Anantaworasakul
title Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract
title_short Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract
title_full Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract
title_fullStr Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract
title_full_unstemmed Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract
title_sort biological activities and antibacterial biomarker of sesbania grandiflora bark extract
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85032663481&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43691
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