Liraglutide preserves intracellular calcium handling in isolated murine myocytes exposed to oxidative stress
© 2017 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. In ischemic/reperfusion (I/R) injured hearts, severe oxidative stress occurs and is associated with intracellular calcium (Ca 2+ ) overload. Glucagon-Like Peptide-1 (GLP-1) analogues have been shown to exert car...
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| Main Authors: | , , |
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| Format: | Journal |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85032960644&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43709 |
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| Institution: | Chiang Mai University |
| Summary: | © 2017 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. In ischemic/reperfusion (I/R) injured hearts, severe oxidative stress occurs and is associated with intracellular calcium (Ca 2+ ) overload. Glucagon-Like Peptide-1 (GLP-1) analogues have been shown to exert cardioprotection in I/R heart. However, there is little information regarding the effects of GLP-1 analogue on the intracellular Ca 2+ regulation in the presence of oxidative stress. Therefore, we investigated the effects of GLP-1 analogue, (liraglutide, 10 μM) applied before or after hydrogen peroxide (H 2 O 2 , 50 μM) treatment on intracellular Ca 2+ regulation in isolated cardiomyocytes. We hypothesized that liraglutide can attenuate intracellular Ca 2+ overload in cardiomyocytes under H 2 O 2 -induced cardiomyocyte injury. Cardiomyocytes were isolated from the hearts of male Wistar rats. Isolated cardiomyocytes were loaded with Fura-2/AM and fluorescence intensity was recorded. Intracellular Ca 2+ transient decay rate, intracellular Ca 2+ transient amplitude and intracellular diastolic Ca 2+ levels were recorded before and after treatment with liraglutide. In H 2 O 2 induced severe oxidative stressed cardiomyocytes (which mimic cardiac I/R) injury, liraglutide given prior to or after H 2 O 2 administration effectively increased both intracellular Ca 2+ transient amplitude and intracellular Ca 2+ transient decay rate, without altering the intracellular diastolic Ca 2+ level. Liraglutide attenuated intracellular Ca 2+ overload in H 2 O 2 -induced cardiomyocyte injury and may be responsible for cardioprotection during cardiac I/R injury by preserving physiological levels of calcium handling during the systolic and diastolic phases of myocyte activation. |
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