Overexpression of ADAM9 in oral squamous cell carcinoma

© 2018, Spandidos Publications. All rights reserved. Overexpression of a disintegrin and metalloproteinase 9 (ADAM9) has been shown in various types of cancer. Some studies have reported inconclusive findings regarding chromosomal aberrations in the ADAM9‑containing region and ADAM9 expression in or...

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Main Authors: Pattaramon Tanasubsinn, Win Pa Pa Aung, Supansa Pata, Witida Laopajon, Anupong Makeudom, Thanapat Sastraruji, Watchara Kasinrerk, Suttichai Krisanaprakornkit
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85035322686&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43908
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spelling th-cmuir.6653943832-439082018-01-24T04:15:01Z Overexpression of ADAM9 in oral squamous cell carcinoma Pattaramon Tanasubsinn Win Pa Pa Aung Supansa Pata Witida Laopajon Anupong Makeudom Thanapat Sastraruji Watchara Kasinrerk Suttichai Krisanaprakornkit © 2018, Spandidos Publications. All rights reserved. Overexpression of a disintegrin and metalloproteinase 9 (ADAM9) has been shown in various types of cancer. Some studies have reported inconclusive findings regarding chromosomal aberrations in the ADAM9‑containing region and ADAM9 expression in oral cancer. Therefore, in this study, ADAM9 protein expression was determined and compared between oral squamous cell carcinoma (OSCC) and normal oral tissues, and between oral cancer cell lines and human oral keratinocytes (HOKs). In total, 34 OSCC and 10 healthy paraffin‑embedded tissue sections were probed with an anti‑ADAM9 antibody, and the immunohistochemical score was determined by multiplying the percentage of positively stained cells with the intensity score. Four different oral cancer and eight independent HOK cell lines were cultured, and the expression of membrane ADAM9 and active ADAM9 at 84 kDa in these cell lines was assayed by flow cytometry and western blot hybridization, respectively. The results showed that the median immunohistochemical score of ADAM9 expression in OSCC tissues was significantly greater than that in normal tissues (P < 0.001). Furthermore, among OSCC cases, intense staining of ADAM9 expression was detected in well‑differentiated and in moderately‑differentiated OSCC; ADAM9 expression was also correlated with an increased degree of cell differentiation (r=0.557; P=0.001). Expression of membrane ADAM9 was present in 3/4 cancer cell lines. Expression of active ADAM9 varied among all the tested cell lines, but significantly higher ADAM9 expression was present in certain cancer cell lines than those in HOKs (P < 0.05). In summary, ADAM9 expression is enhanced in OSCC and oral cancer cell lines, suggesting its role in the pathogenesis of oral cancer. Similar to the overexpression of ADAM9 in well‑differentiated prostate cancer, high degrees of ADAM9 expression have also been observed in well‑differentiated OSCC. 2018-01-24T04:15:01Z 2018-01-24T04:15:01Z 2018-01-01 Journal 17921082 17921074 2-s2.0-85035322686 10.3892/ol.2017.7284 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85035322686&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43908
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2018, Spandidos Publications. All rights reserved. Overexpression of a disintegrin and metalloproteinase 9 (ADAM9) has been shown in various types of cancer. Some studies have reported inconclusive findings regarding chromosomal aberrations in the ADAM9‑containing region and ADAM9 expression in oral cancer. Therefore, in this study, ADAM9 protein expression was determined and compared between oral squamous cell carcinoma (OSCC) and normal oral tissues, and between oral cancer cell lines and human oral keratinocytes (HOKs). In total, 34 OSCC and 10 healthy paraffin‑embedded tissue sections were probed with an anti‑ADAM9 antibody, and the immunohistochemical score was determined by multiplying the percentage of positively stained cells with the intensity score. Four different oral cancer and eight independent HOK cell lines were cultured, and the expression of membrane ADAM9 and active ADAM9 at 84 kDa in these cell lines was assayed by flow cytometry and western blot hybridization, respectively. The results showed that the median immunohistochemical score of ADAM9 expression in OSCC tissues was significantly greater than that in normal tissues (P < 0.001). Furthermore, among OSCC cases, intense staining of ADAM9 expression was detected in well‑differentiated and in moderately‑differentiated OSCC; ADAM9 expression was also correlated with an increased degree of cell differentiation (r=0.557; P=0.001). Expression of membrane ADAM9 was present in 3/4 cancer cell lines. Expression of active ADAM9 varied among all the tested cell lines, but significantly higher ADAM9 expression was present in certain cancer cell lines than those in HOKs (P < 0.05). In summary, ADAM9 expression is enhanced in OSCC and oral cancer cell lines, suggesting its role in the pathogenesis of oral cancer. Similar to the overexpression of ADAM9 in well‑differentiated prostate cancer, high degrees of ADAM9 expression have also been observed in well‑differentiated OSCC.
format Journal
author Pattaramon Tanasubsinn
Win Pa Pa Aung
Supansa Pata
Witida Laopajon
Anupong Makeudom
Thanapat Sastraruji
Watchara Kasinrerk
Suttichai Krisanaprakornkit
spellingShingle Pattaramon Tanasubsinn
Win Pa Pa Aung
Supansa Pata
Witida Laopajon
Anupong Makeudom
Thanapat Sastraruji
Watchara Kasinrerk
Suttichai Krisanaprakornkit
Overexpression of ADAM9 in oral squamous cell carcinoma
author_facet Pattaramon Tanasubsinn
Win Pa Pa Aung
Supansa Pata
Witida Laopajon
Anupong Makeudom
Thanapat Sastraruji
Watchara Kasinrerk
Suttichai Krisanaprakornkit
author_sort Pattaramon Tanasubsinn
title Overexpression of ADAM9 in oral squamous cell carcinoma
title_short Overexpression of ADAM9 in oral squamous cell carcinoma
title_full Overexpression of ADAM9 in oral squamous cell carcinoma
title_fullStr Overexpression of ADAM9 in oral squamous cell carcinoma
title_full_unstemmed Overexpression of ADAM9 in oral squamous cell carcinoma
title_sort overexpression of adam9 in oral squamous cell carcinoma
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85035322686&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43908
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