In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds

© 2015 Taylor and Francis. Porous poly(glycerol sebacate) (PGS) scaffolds were prepared using a salt leaching technique and subsequently surface modified by a low oxygen plasma treatment prior to the use in the in vitro culture of human chondrocytes. Condensation polymerization of glycerol and sebac...

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Main Authors: Tharinee Theerathanagorn, Jeerawan Klangjorhor, Morakot Sakulsombat, Peraphan Pothacharoen, Dumnoensun Pruksakorn, Prachya Kongtawelert, Wanida Janvikul
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/43981
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spelling th-cmuir.6653943832-439812018-04-25T07:44:24Z In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds Tharinee Theerathanagorn Jeerawan Klangjorhor Morakot Sakulsombat Peraphan Pothacharoen Dumnoensun Pruksakorn Prachya Kongtawelert Wanida Janvikul Agricultural and Biological Sciences © 2015 Taylor and Francis. Porous poly(glycerol sebacate) (PGS) scaffolds were prepared using a salt leaching technique and subsequently surface modified by a low oxygen plasma treatment prior to the use in the in vitro culture of human chondrocytes. Condensation polymerization of glycerol and sebacic acid used at various mole ratios, i.e. 1:1, 1:1.25, and 1:1.5, was initially conducted to prepare PGS prepolymers. Porous elastomeric PGS scaffolds were directly fabricated from the mixtures of each prepolymer and 90% (w/w) NaCl particles and then subjected to the plasma treatment to enhance the surface hydrophilicity of the materials. The properties of both untreated and plasma-treated PGS scaffolds were comparatively evaluated, in terms of surface morphology, surface chemical composition, porosity, and storage modulus using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy, micro-computed tomography, and dynamic mechanical analysis, respectively. The responses of chondrocytes cultured on individual PGS scaffolds were assessed, in terms of cell proliferation and ECM production. The results revealed that average pore sizes and porosity of the scaffolds were increased with an increasing sebacic acid concentration used. The storage moduli of the scaffolds were raised after the plasma treatment, possibly due to the further crosslinking of PGS upon treatment. Moreover, the scaffold prepared with a higher sebacic acid content demonstrated a greater capability of promoting cell infiltration, proliferation, and ECM production, especially when it was plasma-treated; the greatest HA, sGAG, uronic acid, and collagen contents were detected in matrix of this scaffold. The H & E and safranin O staining results also strongly supported this finding. The storage modulus of the scaffold was intensified after incubation with the chondrocytes for 21 days, indicating the accretion and retention of matrix ECM on the cell-cultured scaffold. 2018-01-24T04:36:45Z 2018-01-24T04:36:45Z 2015-12-12 Journal 15685624 09205063 2-s2.0-84947612814 10.1080/09205063.2015.1096446 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84947612814&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43981
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Tharinee Theerathanagorn
Jeerawan Klangjorhor
Morakot Sakulsombat
Peraphan Pothacharoen
Dumnoensun Pruksakorn
Prachya Kongtawelert
Wanida Janvikul
In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds
description © 2015 Taylor and Francis. Porous poly(glycerol sebacate) (PGS) scaffolds were prepared using a salt leaching technique and subsequently surface modified by a low oxygen plasma treatment prior to the use in the in vitro culture of human chondrocytes. Condensation polymerization of glycerol and sebacic acid used at various mole ratios, i.e. 1:1, 1:1.25, and 1:1.5, was initially conducted to prepare PGS prepolymers. Porous elastomeric PGS scaffolds were directly fabricated from the mixtures of each prepolymer and 90% (w/w) NaCl particles and then subjected to the plasma treatment to enhance the surface hydrophilicity of the materials. The properties of both untreated and plasma-treated PGS scaffolds were comparatively evaluated, in terms of surface morphology, surface chemical composition, porosity, and storage modulus using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy, micro-computed tomography, and dynamic mechanical analysis, respectively. The responses of chondrocytes cultured on individual PGS scaffolds were assessed, in terms of cell proliferation and ECM production. The results revealed that average pore sizes and porosity of the scaffolds were increased with an increasing sebacic acid concentration used. The storage moduli of the scaffolds were raised after the plasma treatment, possibly due to the further crosslinking of PGS upon treatment. Moreover, the scaffold prepared with a higher sebacic acid content demonstrated a greater capability of promoting cell infiltration, proliferation, and ECM production, especially when it was plasma-treated; the greatest HA, sGAG, uronic acid, and collagen contents were detected in matrix of this scaffold. The H & E and safranin O staining results also strongly supported this finding. The storage modulus of the scaffold was intensified after incubation with the chondrocytes for 21 days, indicating the accretion and retention of matrix ECM on the cell-cultured scaffold.
format Journal
author Tharinee Theerathanagorn
Jeerawan Klangjorhor
Morakot Sakulsombat
Peraphan Pothacharoen
Dumnoensun Pruksakorn
Prachya Kongtawelert
Wanida Janvikul
author_facet Tharinee Theerathanagorn
Jeerawan Klangjorhor
Morakot Sakulsombat
Peraphan Pothacharoen
Dumnoensun Pruksakorn
Prachya Kongtawelert
Wanida Janvikul
author_sort Tharinee Theerathanagorn
title In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds
title_short In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds
title_full In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds
title_fullStr In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds
title_full_unstemmed In vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds
title_sort in vitro human chondrocyte culture on plasma-treated poly(glycerol sebacate) scaffolds
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84947612814&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43981
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