Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants

© 2015 Siengdee et al. Background: Intra-articular injection of corticosteroids is used to treat the inflammatory pain of arthritis and osteoarthritis (OA), but our previous study found a deleterious effect of these steroids on chondrocyte cells. Hyaluronic acid (HA) injection has been suggested as...

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Main Authors: Puntita Siengdee, Tiwaporn Radeerom, Similan Kuanoon, Thippaporn Euppayo, Waranee Pradit, Siriwadee Chomdej, Siriwan Ongchai, Korakot Nganvongpanit
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/43990
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spelling th-cmuir.6653943832-439902018-04-25T07:44:27Z Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants Puntita Siengdee Tiwaporn Radeerom Similan Kuanoon Thippaporn Euppayo Waranee Pradit Siriwadee Chomdej Siriwan Ongchai Korakot Nganvongpanit Agricultural and Biological Sciences © 2015 Siengdee et al. Background: Intra-articular injection of corticosteroids is used to treat the inflammatory pain of arthritis and osteoarthritis (OA), but our previous study found a deleterious effect of these steroids on chondrocyte cells. Hyaluronic acid (HA) injection has been suggested as a means to counteract negative side effects through replenishment of synovial fluid that can decrease pain in affected joints. To better understand the effects of corticosteroids on these processes, dexamethasone (Dex) and prednisolone (Pred) were administered to porcine cartilage explants at several concentrations with and without HA. We examined corticoid effects by determining sulfate-glycosaminoglycan (s-GAG) and uronic acid (UA) content of the explant media, and safranin-O staining of the cells. Analysis of lactate dehydrogenase (LDH) activity was conducted to assess cell cytotoxicity. Results: Dex treatment significantly reduced cellular cytotoxicity compared to the other treatment groups, especially with regards to the release of s-GAG, and protects against superficial proteoglycan damage. However, there was no difference between Pred and Dex, with and without HA, in the UA content remaining in porcine cartilage explants. Conclusions: The data suggest that combinations of Dex and Pred with HA did not have a significant effect on protection or enhancement of the articular cartilage matrix under the current conditions. 2018-01-24T04:36:50Z 2018-01-24T04:36:50Z 2015-12-04 Journal 17466148 2-s2.0-84949238562 10.1186/s12917-015-0611-6 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84949238562&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/43990
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Puntita Siengdee
Tiwaporn Radeerom
Similan Kuanoon
Thippaporn Euppayo
Waranee Pradit
Siriwadee Chomdej
Siriwan Ongchai
Korakot Nganvongpanit
Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants
description © 2015 Siengdee et al. Background: Intra-articular injection of corticosteroids is used to treat the inflammatory pain of arthritis and osteoarthritis (OA), but our previous study found a deleterious effect of these steroids on chondrocyte cells. Hyaluronic acid (HA) injection has been suggested as a means to counteract negative side effects through replenishment of synovial fluid that can decrease pain in affected joints. To better understand the effects of corticosteroids on these processes, dexamethasone (Dex) and prednisolone (Pred) were administered to porcine cartilage explants at several concentrations with and without HA. We examined corticoid effects by determining sulfate-glycosaminoglycan (s-GAG) and uronic acid (UA) content of the explant media, and safranin-O staining of the cells. Analysis of lactate dehydrogenase (LDH) activity was conducted to assess cell cytotoxicity. Results: Dex treatment significantly reduced cellular cytotoxicity compared to the other treatment groups, especially with regards to the release of s-GAG, and protects against superficial proteoglycan damage. However, there was no difference between Pred and Dex, with and without HA, in the UA content remaining in porcine cartilage explants. Conclusions: The data suggest that combinations of Dex and Pred with HA did not have a significant effect on protection or enhancement of the articular cartilage matrix under the current conditions.
format Journal
author Puntita Siengdee
Tiwaporn Radeerom
Similan Kuanoon
Thippaporn Euppayo
Waranee Pradit
Siriwadee Chomdej
Siriwan Ongchai
Korakot Nganvongpanit
author_facet Puntita Siengdee
Tiwaporn Radeerom
Similan Kuanoon
Thippaporn Euppayo
Waranee Pradit
Siriwadee Chomdej
Siriwan Ongchai
Korakot Nganvongpanit
author_sort Puntita Siengdee
title Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants
title_short Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants
title_full Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants
title_fullStr Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants
title_full_unstemmed Effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants
title_sort effects of corticosteroids and their combinations with hyaluronanon on the biochemical properties of porcine cartilage explants
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84949238562&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/43990
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