Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones
© 2015, Medical Association of Thailand. All rights reserved. Objective: To develop a method for human genomic DNA extraction from bone using gelatin-coated magnetic particles. Material and Method: Thirty human metacarpal with the bone age ranging from 36 to 93 years were included in the present stu...
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th-cmuir.6653943832-445142018-04-25T07:51:28Z Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones Pongsak Khanpetch Sorasak Intorasoot Sukon Prasitwattanseree Karnda Mekjaidee Pasuk Mahakkanukrauh Agricultural and Biological Sciences © 2015, Medical Association of Thailand. All rights reserved. Objective: To develop a method for human genomic DNA extraction from bone using gelatin-coated magnetic particles. Material and Method: Thirty human metacarpal with the bone age ranging from 36 to 93 years were included in the present study. Genomic DNA was extracted from bones using gelatin-coated magnetic particles. The concentration and purity of DNA were analyzed in comparison with a reference method. In addition, the quality of extracted DNA was examined for sex determination by conventional polymerase chain reaction (PCR). Results: The average DNA concentration using gelatin coated magnetic particles exhibited approximately 15 times higher than a reference method with an insignificantly difference of the DNA purity in both methods. Twelve (40%) and fifteen (50%) samples out of thirty DNA isolated using established and reference method, respectively, could be amplified and sex correctly determined by PCR. Conclusion: Gelatin coated magnetic particle is rapid, simple, and well-suited for isolation of DNA from bones. 2018-01-24T04:44:03Z 2018-01-24T04:44:03Z 2015-01-01 Journal 01252208 01252208 2-s2.0-84938093041 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84938093041&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/44514 |
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Agricultural and Biological Sciences Pongsak Khanpetch Sorasak Intorasoot Sukon Prasitwattanseree Karnda Mekjaidee Pasuk Mahakkanukrauh Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones |
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© 2015, Medical Association of Thailand. All rights reserved. Objective: To develop a method for human genomic DNA extraction from bone using gelatin-coated magnetic particles. Material and Method: Thirty human metacarpal with the bone age ranging from 36 to 93 years were included in the present study. Genomic DNA was extracted from bones using gelatin-coated magnetic particles. The concentration and purity of DNA were analyzed in comparison with a reference method. In addition, the quality of extracted DNA was examined for sex determination by conventional polymerase chain reaction (PCR). Results: The average DNA concentration using gelatin coated magnetic particles exhibited approximately 15 times higher than a reference method with an insignificantly difference of the DNA purity in both methods. Twelve (40%) and fifteen (50%) samples out of thirty DNA isolated using established and reference method, respectively, could be amplified and sex correctly determined by PCR. Conclusion: Gelatin coated magnetic particle is rapid, simple, and well-suited for isolation of DNA from bones. |
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Journal |
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Pongsak Khanpetch Sorasak Intorasoot Sukon Prasitwattanseree Karnda Mekjaidee Pasuk Mahakkanukrauh |
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Pongsak Khanpetch Sorasak Intorasoot Sukon Prasitwattanseree Karnda Mekjaidee Pasuk Mahakkanukrauh |
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Pongsak Khanpetch |
title |
Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones |
title_short |
Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones |
title_full |
Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones |
title_fullStr |
Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones |
title_full_unstemmed |
Application of gelatin-coated magnetic particles for isolation of genomic DNA from bones |
title_sort |
application of gelatin-coated magnetic particles for isolation of genomic dna from bones |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84938093041&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/44514 |
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