Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor

© 2014 Elsevier Ltd. The viral levels and immune responses of Italian honey bees (IHB), Russian honey bees (RHB) and an outcross of Varroa Sensitive Hygienic bees (POL) deliberately infested with one or two foundress Varroa were compared. We found that the Deformed wing virus (DWV) level in IHB inoc...

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Main Authors: Kitiphong Khongphinitbunjong, Lilia I. de Guzman, Matthew R. Tarver, Thomas E. Rinderer, Yanping Chen, Panuwan Chantawannakul
Format: Journal
Published: 2018
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-448492018-04-25T07:55:55Z Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor Kitiphong Khongphinitbunjong Lilia I. de Guzman Matthew R. Tarver Thomas E. Rinderer Yanping Chen Panuwan Chantawannakul Agricultural and Biological Sciences © 2014 Elsevier Ltd. The viral levels and immune responses of Italian honey bees (IHB), Russian honey bees (RHB) and an outcross of Varroa Sensitive Hygienic bees (POL) deliberately infested with one or two foundress Varroa were compared. We found that the Deformed wing virus (DWV) level in IHB inoculated with one or two foundress Varroa increased to about 10 3 or 10 5 fold the levels of their uninfested brood. In contrast, POL (10 2 or 10 4 fold) and RHB (10 2 or l0 4 fold) supported a lower increase in DWV levels. The feeding of different stages of Varroa nymphs did not increase DWV levels of their pupal hosts. Analyses of their corresponding Varroa mites showed the same trends: two foundress Varroa yielded higher DWV levels than one foundress, and the addition of nymphs did not increase viral levels. Using the same pupae examined for the presence of viruses, 16 out of 24 genes evaluated showed significant differential mRNA expression levels among the three honey bee stocks. However, only four genes (Defensin, Dscam, PPOact and spaetzle), which were expressed at similar levels in uninfested pupae, were altered by the number of feeding foundress Varroa and levels of DWV regardless of stocks. This research provides the first evidence that immune response profiles of different honey bee stocks are induced by Varroa parasitism. 2018-01-24T04:48:57Z 2018-01-24T04:48:57Z 2015-01-01 Journal 00221910 2-s2.0-84949117006 10.1016/j.jinsphys.2014.11.005 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84949117006&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/44849
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Kitiphong Khongphinitbunjong
Lilia I. de Guzman
Matthew R. Tarver
Thomas E. Rinderer
Yanping Chen
Panuwan Chantawannakul
Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor
description © 2014 Elsevier Ltd. The viral levels and immune responses of Italian honey bees (IHB), Russian honey bees (RHB) and an outcross of Varroa Sensitive Hygienic bees (POL) deliberately infested with one or two foundress Varroa were compared. We found that the Deformed wing virus (DWV) level in IHB inoculated with one or two foundress Varroa increased to about 10 3 or 10 5 fold the levels of their uninfested brood. In contrast, POL (10 2 or 10 4 fold) and RHB (10 2 or l0 4 fold) supported a lower increase in DWV levels. The feeding of different stages of Varroa nymphs did not increase DWV levels of their pupal hosts. Analyses of their corresponding Varroa mites showed the same trends: two foundress Varroa yielded higher DWV levels than one foundress, and the addition of nymphs did not increase viral levels. Using the same pupae examined for the presence of viruses, 16 out of 24 genes evaluated showed significant differential mRNA expression levels among the three honey bee stocks. However, only four genes (Defensin, Dscam, PPOact and spaetzle), which were expressed at similar levels in uninfested pupae, were altered by the number of feeding foundress Varroa and levels of DWV regardless of stocks. This research provides the first evidence that immune response profiles of different honey bee stocks are induced by Varroa parasitism.
format Journal
author Kitiphong Khongphinitbunjong
Lilia I. de Guzman
Matthew R. Tarver
Thomas E. Rinderer
Yanping Chen
Panuwan Chantawannakul
author_facet Kitiphong Khongphinitbunjong
Lilia I. de Guzman
Matthew R. Tarver
Thomas E. Rinderer
Yanping Chen
Panuwan Chantawannakul
author_sort Kitiphong Khongphinitbunjong
title Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor
title_short Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor
title_full Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor
title_fullStr Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor
title_full_unstemmed Differential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructor
title_sort differential viral levels and immune gene expression in three stocks of apis mellifera induced by different numbers of varroa destructor
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84949117006&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/44849
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