Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L

IUMS. The taxonomic status of a rhizospheric soil actinomycete, designated R8-39(T), was established using a polyphasic approach. The organism had phenotypic and morphological characteristics consistent with its classification in the genus Allokutzneria. Phylogenetic analysis based on an almost comp...

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Main Authors: Kannika Duangmal, Nalin Poomthongdee, Wasu Pathom-aree, Akira Takè, Arinthip Thamchaipenet, Atsuko Matsumoto, Yoko Takahashi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/44957
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spelling th-cmuir.6653943832-449572018-01-24T06:02:01Z Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L Kannika Duangmal Nalin Poomthongdee Wasu Pathom-aree Akira Takè Arinthip Thamchaipenet Atsuko Matsumoto Yoko Takahashi IUMS. The taxonomic status of a rhizospheric soil actinomycete, designated R8-39(T), was established using a polyphasic approach. The organism had phenotypic and morphological characteristics consistent with its classification in the genus Allokutzneria. Phylogenetic analysis based on an almost complete 16S rRNA gene sequence showed that the strain formed a monophyletic clade with the type strains of members of the genus Allokutzneria. Strain R8-39(T) displayed the highest levels of 16S rRNA gene sequence similarity to Allokutzneria albata DSM 44149(T) (98.8%) and Allokutzneria multivorans YIM 120521(T) (98.3%). However, the DNA-DNA hybridization values between strain R8-39(T) and A. albata and A. multivorans were clearly below the 70% threshold. The organism was found to have chemical characteristics consistent with its classification in the genus Allokutzneria. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, mannose, rhamnose and ribose. The main menaquinone was MK-9(H4). No mycolic acid was detected. The G+C content of the genomic DNA was 71.8 mol%. In addition, strain R8-39(T) had a phenotypic profile that readily distinguished it from recognized representatives of the genus Allokutzneria. It is evident from the combined genotypic and phenotypic properties that strain R8-39(T) represents a novel species of the genus Allokutzneria. The proposed name for this species is Allokutzneria oryzae sp. nov.; the type strain is R8-39(T) ( = BCC 60399(T) = NBRC 109649(T)). 2018-01-24T06:02:01Z 2018-01-24T06:02:01Z 2014-10-01 Journal 14665034 2-s2.0-84921964841 10.1099/ijs.0.065169-0 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84921964841&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/44957
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description IUMS. The taxonomic status of a rhizospheric soil actinomycete, designated R8-39(T), was established using a polyphasic approach. The organism had phenotypic and morphological characteristics consistent with its classification in the genus Allokutzneria. Phylogenetic analysis based on an almost complete 16S rRNA gene sequence showed that the strain formed a monophyletic clade with the type strains of members of the genus Allokutzneria. Strain R8-39(T) displayed the highest levels of 16S rRNA gene sequence similarity to Allokutzneria albata DSM 44149(T) (98.8%) and Allokutzneria multivorans YIM 120521(T) (98.3%). However, the DNA-DNA hybridization values between strain R8-39(T) and A. albata and A. multivorans were clearly below the 70% threshold. The organism was found to have chemical characteristics consistent with its classification in the genus Allokutzneria. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, mannose, rhamnose and ribose. The main menaquinone was MK-9(H4). No mycolic acid was detected. The G+C content of the genomic DNA was 71.8 mol%. In addition, strain R8-39(T) had a phenotypic profile that readily distinguished it from recognized representatives of the genus Allokutzneria. It is evident from the combined genotypic and phenotypic properties that strain R8-39(T) represents a novel species of the genus Allokutzneria. The proposed name for this species is Allokutzneria oryzae sp. nov.; the type strain is R8-39(T) ( = BCC 60399(T) = NBRC 109649(T)).
format Journal
author Kannika Duangmal
Nalin Poomthongdee
Wasu Pathom-aree
Akira Takè
Arinthip Thamchaipenet
Atsuko Matsumoto
Yoko Takahashi
spellingShingle Kannika Duangmal
Nalin Poomthongdee
Wasu Pathom-aree
Akira Takè
Arinthip Thamchaipenet
Atsuko Matsumoto
Yoko Takahashi
Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L
author_facet Kannika Duangmal
Nalin Poomthongdee
Wasu Pathom-aree
Akira Takè
Arinthip Thamchaipenet
Atsuko Matsumoto
Yoko Takahashi
author_sort Kannika Duangmal
title Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L
title_short Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L
title_full Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L
title_fullStr Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L
title_full_unstemmed Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L
title_sort allokutzneria oryzae sp. nov., isolated from rhizospheric soil of oryza sativa l
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84921964841&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/44957
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