A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer

A novel chemiluminescence (CL) microfluidic system incorporating a molecularly imprinted polymer (MIP) preconcentration step was used for the determination of chloramphenicol in honey samples. The MIP was prepared by using chloramphenicol as the template, diethylaminoethyl methacrylate (DAM) as the...

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Main Authors: Thongchai W., Liawruangath B., Liawruangrath S., Greenway G.M.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-77955321873&partnerID=40&md5=8fc34c50cd9a317aa3dff0c081dca882
http://www.ncbi.nlm.nih.gov/pubmed/20602936
http://cmuir.cmu.ac.th/handle/6653943832/4497
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spelling th-cmuir.6653943832-44972014-08-30T02:42:30Z A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer Thongchai W. Liawruangath B. Liawruangrath S. Greenway G.M. A novel chemiluminescence (CL) microfluidic system incorporating a molecularly imprinted polymer (MIP) preconcentration step was used for the determination of chloramphenicol in honey samples. The MIP was prepared by using chloramphenicol as the template, diethylaminoethyl methacrylate (DAM) as the function monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, 2, 2′-dimethoxy-2-phenylacetophenone (DMPA) as the free radical initiator and toluene and dodecanol as the solvent. The MIP was pre-loaded into a 10 mm long, 2 mm wide and 150 μm deep channel in a planar glass microfluidic device. When the sample containing chloramphenicol was introduced into the microfluidic device it was first preconcentrated on the MIP then detected by an enhancement effect on the chemiluminescence reaction of tris(2, 2′-bipyridyl) ruthenium(II) with cerium(IV) sulphate in sulphuric acid. A micro-syringe pump was used to pump the reagents. The CL intensity was linear in relationship to the chloramphenicol concentrations from 1.55 × 10 -4 to 3.09 × 10-3 μmol L-1 (r 2 = 0.9915) and the detection limit (3σ) and the quantitation limit (10σ) were found to be 7.46 × 10-6 and 2.48 × 10-5 μmol L-1, respectively. This method offered a high selectivity and sensitivity for quantitative analysis of chloramphenicol in the honey samples. © 2010 Elsevier B.V. All rights reserved. 2014-08-30T02:42:30Z 2014-08-30T02:42:30Z 2010 Article 399140 10.1016/j.talanta.2010.05.007 20602936 TLNTA http://www.scopus.com/inward/record.url?eid=2-s2.0-77955321873&partnerID=40&md5=8fc34c50cd9a317aa3dff0c081dca882 http://www.ncbi.nlm.nih.gov/pubmed/20602936 http://cmuir.cmu.ac.th/handle/6653943832/4497 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A novel chemiluminescence (CL) microfluidic system incorporating a molecularly imprinted polymer (MIP) preconcentration step was used for the determination of chloramphenicol in honey samples. The MIP was prepared by using chloramphenicol as the template, diethylaminoethyl methacrylate (DAM) as the function monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, 2, 2′-dimethoxy-2-phenylacetophenone (DMPA) as the free radical initiator and toluene and dodecanol as the solvent. The MIP was pre-loaded into a 10 mm long, 2 mm wide and 150 μm deep channel in a planar glass microfluidic device. When the sample containing chloramphenicol was introduced into the microfluidic device it was first preconcentrated on the MIP then detected by an enhancement effect on the chemiluminescence reaction of tris(2, 2′-bipyridyl) ruthenium(II) with cerium(IV) sulphate in sulphuric acid. A micro-syringe pump was used to pump the reagents. The CL intensity was linear in relationship to the chloramphenicol concentrations from 1.55 × 10 -4 to 3.09 × 10-3 μmol L-1 (r 2 = 0.9915) and the detection limit (3σ) and the quantitation limit (10σ) were found to be 7.46 × 10-6 and 2.48 × 10-5 μmol L-1, respectively. This method offered a high selectivity and sensitivity for quantitative analysis of chloramphenicol in the honey samples. © 2010 Elsevier B.V. All rights reserved.
format Article
author Thongchai W.
Liawruangath B.
Liawruangrath S.
Greenway G.M.
spellingShingle Thongchai W.
Liawruangath B.
Liawruangrath S.
Greenway G.M.
A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
author_facet Thongchai W.
Liawruangath B.
Liawruangrath S.
Greenway G.M.
author_sort Thongchai W.
title A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
title_short A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
title_full A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
title_fullStr A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
title_full_unstemmed A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
title_sort microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-77955321873&partnerID=40&md5=8fc34c50cd9a317aa3dff0c081dca882
http://www.ncbi.nlm.nih.gov/pubmed/20602936
http://cmuir.cmu.ac.th/handle/6653943832/4497
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