Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species

The development of sequential injection analysis with lab-at-valve (LAV) semi-automated system on-line liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various Solanum species fruits. The main proposed is semi-automated extractive determination of solaso...

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Main Authors: Thongchai W., Liawruangrath B., Liawruangrath S.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-77049123431&partnerID=40&md5=108fbad16a6c760691943bdf419a1071
http://www.ncbi.nlm.nih.gov/pubmed/20188963
http://cmuir.cmu.ac.th/handle/6653943832/4506
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-45062014-08-30T02:42:31Z Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species Thongchai W. Liawruangrath B. Liawruangrath S. The development of sequential injection analysis with lab-at-valve (LAV) semi-automated system on-line liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various Solanum species fruits. The main proposed is semi-automated extractive determination of solasodine using methyl orange as colorimetric reagent. After optimization of the system, sample, reagent and organic solvent were sequentially aspirated into an extraction coil connected to the center of a selection valve, where extraction took place by flow reversal. The aqueous and organic phases were separated in a lab-at-valve unit attracted to one of the ports of the selection valve. The absorption of ion-pair solasodine-methyl orange complex in the organic phase was measured spectrophotometrically at 420 nm. The method performances, including reproducibility, linearity, sensitivity and accuracy, were also evaluated. The proposed method is simple, reproducible and accurate. It was successfully applied to the determination of solasodine in Solanum aculeatissimum Jacq., Solanum violaceum Ortega., Solanum melongena Linn. and Solanum indicum Linn. fruits in Solanaceae family. Results obtained were in good agreement with those obtained by batch wise spectrophotometric method. It is also suitable and useful for determination of solasodine in other medicinal plants. © 2009 Elsevier B.V. All rights reserved. 2014-08-30T02:42:31Z 2014-08-30T02:42:31Z 2010 Article 399140 10.1016/j.talanta.2009.12.044 20188963 TLNTA http://www.scopus.com/inward/record.url?eid=2-s2.0-77049123431&partnerID=40&md5=108fbad16a6c760691943bdf419a1071 http://www.ncbi.nlm.nih.gov/pubmed/20188963 http://cmuir.cmu.ac.th/handle/6653943832/4506 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The development of sequential injection analysis with lab-at-valve (LAV) semi-automated system on-line liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various Solanum species fruits. The main proposed is semi-automated extractive determination of solasodine using methyl orange as colorimetric reagent. After optimization of the system, sample, reagent and organic solvent were sequentially aspirated into an extraction coil connected to the center of a selection valve, where extraction took place by flow reversal. The aqueous and organic phases were separated in a lab-at-valve unit attracted to one of the ports of the selection valve. The absorption of ion-pair solasodine-methyl orange complex in the organic phase was measured spectrophotometrically at 420 nm. The method performances, including reproducibility, linearity, sensitivity and accuracy, were also evaluated. The proposed method is simple, reproducible and accurate. It was successfully applied to the determination of solasodine in Solanum aculeatissimum Jacq., Solanum violaceum Ortega., Solanum melongena Linn. and Solanum indicum Linn. fruits in Solanaceae family. Results obtained were in good agreement with those obtained by batch wise spectrophotometric method. It is also suitable and useful for determination of solasodine in other medicinal plants. © 2009 Elsevier B.V. All rights reserved.
format Article
author Thongchai W.
Liawruangrath B.
Liawruangrath S.
spellingShingle Thongchai W.
Liawruangrath B.
Liawruangrath S.
Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species
author_facet Thongchai W.
Liawruangrath B.
Liawruangrath S.
author_sort Thongchai W.
title Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species
title_short Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species
title_full Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species
title_fullStr Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species
title_full_unstemmed Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species
title_sort sequential injection analysis with lab-at-valve (si-lav) for the determination of solasodine in solanum species
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-77049123431&partnerID=40&md5=108fbad16a6c760691943bdf419a1071
http://www.ncbi.nlm.nih.gov/pubmed/20188963
http://cmuir.cmu.ac.th/handle/6653943832/4506
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