Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis

Aim To measure the levels of hCAP18/LL-37 in gingival crevicular fluid from patients with periodontal diseases compared with healthy controls and to determine the correlation between hCAP18/LL-37 and chondroitin sulphate (CS) levels in patients with periodontitis. Material and Methods Gingival crevi...

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Main Authors: Anupong Makeudom, Samakorn Kulpawaropas, Pattanin Montreekachon, Sakornrat Khongkhunthian, Thanapat Sastraruji, Peraphan Pothacharoen, Prachya Kongtawelert, Suttichai Krisanaprakornkit
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893507456&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45127
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-451272018-01-24T06:05:42Z Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis Anupong Makeudom Samakorn Kulpawaropas Pattanin Montreekachon Sakornrat Khongkhunthian Thanapat Sastraruji Peraphan Pothacharoen Prachya Kongtawelert Suttichai Krisanaprakornkit Aim To measure the levels of hCAP18/LL-37 in gingival crevicular fluid from patients with periodontal diseases compared with healthy controls and to determine the correlation between hCAP18/LL-37 and chondroitin sulphate (CS) levels in patients with periodontitis. Material and Methods Gingival crevicular fluid samples from 51 patients and 25 healthy volunteers were analysed for the hCAP18/LL-37 levels by immunoblotting and were determined for the CS levels by the competitive enzyme-linked immunosorbent assay. Results Tris buffer pH 9.85 was selected to recover hCAP18/LL-37 from Periopaper strips, in which the percentages of recovery were around 70%. The median levels of hCAP18/LL-37 in the aggressive and the chronic periodontitis (CP) groups were significantly greater than those in the gingivitis and the healthy groups (p < 0.05). Significant correlations between the unprocessed 18-kDa fragment and CS levels (r = 0.650; p < 0.001) and between the mature 4.6-kDa fragment and CS levels (r = 0.502; p < 0.001) were observed only in the CP group. Conclusion The significant correlations between the hCAP18/LL-37 and the CS levels were found in CP, but not in aggressive periodontitis. The presence versus absence of such correlations may be clinically applicable to help clinicians distinguish between two distinct types of periodontitis. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. 2018-01-24T06:05:42Z 2018-01-24T06:05:42Z 2014-03-01 Journal 1600051X 03036979 2-s2.0-84893507456 10.1111/jcpe.12216 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893507456&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/45127
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Aim To measure the levels of hCAP18/LL-37 in gingival crevicular fluid from patients with periodontal diseases compared with healthy controls and to determine the correlation between hCAP18/LL-37 and chondroitin sulphate (CS) levels in patients with periodontitis. Material and Methods Gingival crevicular fluid samples from 51 patients and 25 healthy volunteers were analysed for the hCAP18/LL-37 levels by immunoblotting and were determined for the CS levels by the competitive enzyme-linked immunosorbent assay. Results Tris buffer pH 9.85 was selected to recover hCAP18/LL-37 from Periopaper strips, in which the percentages of recovery were around 70%. The median levels of hCAP18/LL-37 in the aggressive and the chronic periodontitis (CP) groups were significantly greater than those in the gingivitis and the healthy groups (p < 0.05). Significant correlations between the unprocessed 18-kDa fragment and CS levels (r = 0.650; p < 0.001) and between the mature 4.6-kDa fragment and CS levels (r = 0.502; p < 0.001) were observed only in the CP group. Conclusion The significant correlations between the hCAP18/LL-37 and the CS levels were found in CP, but not in aggressive periodontitis. The presence versus absence of such correlations may be clinically applicable to help clinicians distinguish between two distinct types of periodontitis. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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author Anupong Makeudom
Samakorn Kulpawaropas
Pattanin Montreekachon
Sakornrat Khongkhunthian
Thanapat Sastraruji
Peraphan Pothacharoen
Prachya Kongtawelert
Suttichai Krisanaprakornkit
spellingShingle Anupong Makeudom
Samakorn Kulpawaropas
Pattanin Montreekachon
Sakornrat Khongkhunthian
Thanapat Sastraruji
Peraphan Pothacharoen
Prachya Kongtawelert
Suttichai Krisanaprakornkit
Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis
author_facet Anupong Makeudom
Samakorn Kulpawaropas
Pattanin Montreekachon
Sakornrat Khongkhunthian
Thanapat Sastraruji
Peraphan Pothacharoen
Prachya Kongtawelert
Suttichai Krisanaprakornkit
author_sort Anupong Makeudom
title Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis
title_short Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis
title_full Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis
title_fullStr Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis
title_full_unstemmed Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis
title_sort positive correlations between hcap18/ll-37 and chondroitin sulphate levels in chronic periodontitis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893507456&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45127
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