Identification of the haemolytic activity of Malassezia species

Malassezia species are part of the normal skin flora and are associated with a number of human and animal skin diseases. However, the mechanisms that mediate infection and host-fungal interactions are poorly understood. The haemolytic activity of several microorganisms is considered a factor that co...

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Main Authors: Weerapong Juntachai, Aksarakorn Kummasook, Malee Mekaprateep, Susumu Kajiwara
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893737351&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45149
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-451492018-01-24T06:06:01Z Identification of the haemolytic activity of Malassezia species Weerapong Juntachai Aksarakorn Kummasook Malee Mekaprateep Susumu Kajiwara Malassezia species are part of the normal skin flora and are associated with a number of human and animal skin diseases. However, the mechanisms that mediate infection and host-fungal interactions are poorly understood. The haemolytic activity of several microorganisms is considered a factor that contributes to pathogenicity of the organism to humans and animals. This virulence factor was previously identified in several pathogenic fungi that cause systemic mycoses, such as Aspergillus and Candida. In this study, the haemolytic activity of six major Malassezia species, including M. furfur, M. globosa, M. pachydermatis, M. restricta, M. slooffiae and M. sympodialis, was investigated. The haemolytic activity of these species was tested on tryptone soya agar with 5% sheep blood. All the examined Malassezia species produced a halo zone of complete haemolysis. A quantitative analysis of the haemolytic activity was performed by incubating sheep erythrocytes with the extraction from culture of each Malassezia species. Interestingly, M. globosa and M. restricta showed significantly high haemolytic activity compared with the other Malassezia species. In addition, M. globosa also exhibited stable haemolytic activity after treatment at 100 °C and in the presence of some proteases, indicating that this haemolytic factor is different from those of other fungi. © 2013 Blackwell Verlag GmbH. 2018-01-24T06:06:01Z 2018-01-24T06:06:01Z 2014-03-01 Journal 14390507 09337407 2-s2.0-84893737351 10.1111/myc.12125 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893737351&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/45149
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Malassezia species are part of the normal skin flora and are associated with a number of human and animal skin diseases. However, the mechanisms that mediate infection and host-fungal interactions are poorly understood. The haemolytic activity of several microorganisms is considered a factor that contributes to pathogenicity of the organism to humans and animals. This virulence factor was previously identified in several pathogenic fungi that cause systemic mycoses, such as Aspergillus and Candida. In this study, the haemolytic activity of six major Malassezia species, including M. furfur, M. globosa, M. pachydermatis, M. restricta, M. slooffiae and M. sympodialis, was investigated. The haemolytic activity of these species was tested on tryptone soya agar with 5% sheep blood. All the examined Malassezia species produced a halo zone of complete haemolysis. A quantitative analysis of the haemolytic activity was performed by incubating sheep erythrocytes with the extraction from culture of each Malassezia species. Interestingly, M. globosa and M. restricta showed significantly high haemolytic activity compared with the other Malassezia species. In addition, M. globosa also exhibited stable haemolytic activity after treatment at 100 °C and in the presence of some proteases, indicating that this haemolytic factor is different from those of other fungi. © 2013 Blackwell Verlag GmbH.
format Journal
author Weerapong Juntachai
Aksarakorn Kummasook
Malee Mekaprateep
Susumu Kajiwara
spellingShingle Weerapong Juntachai
Aksarakorn Kummasook
Malee Mekaprateep
Susumu Kajiwara
Identification of the haemolytic activity of Malassezia species
author_facet Weerapong Juntachai
Aksarakorn Kummasook
Malee Mekaprateep
Susumu Kajiwara
author_sort Weerapong Juntachai
title Identification of the haemolytic activity of Malassezia species
title_short Identification of the haemolytic activity of Malassezia species
title_full Identification of the haemolytic activity of Malassezia species
title_fullStr Identification of the haemolytic activity of Malassezia species
title_full_unstemmed Identification of the haemolytic activity of Malassezia species
title_sort identification of the haemolytic activity of malassezia species
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893737351&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45149
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