Measurement of HbA<inf>2</inf> by capillary electrophoresis for diagnosing β-thalassemia/HbE disease in patients with low hbf

• Main Authors: Watcharee Prasing, Sakorn Pornprasert
• Format: Journal
• Published: 2018
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84940573467&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/45302
• Institution: Chiang Mai University

Objective: Capillary electrophoresis (CE) has the ability to detect hemoglobin (Hb) A 2 in the presence of HbE. The aim of this study was to compare levels of HbA 2 measured by CE between β-thalassemia/HbE and homozygous HbE patients. Methods: Molecular analysis for diagnosing of β-thalassemia gene mutations and HbE was performed in 28 blood samples containing of HbE > 75% and HbF levels varying from 5% to 15% quantified by highperformance liquid chromatography (HPLC). Levels of HbA 2 , HbE, and HbF were then measured using CE. Results: Nine patients were diagnosed with β-thalassemia/HbE disease and 19 with homozygous HbE. All patients with β-thalassemia/HbE disease and only one patient (5%) with homozygous HbE had HbA 2 higher than 6.0%. Conclusions: The analysis of HbA2 level ( > 6.0%) by CE may be an alternative method for preliminary diagnosis of β-thalassemia/HbE with uncertain β-thalassemia/HbE disease and homozygous HbE.