Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa

The objective of this study was to produce monoclonal antibody (MAb) against Y-bearing spermatozoa. The antibody will be used in cytotoxic immunological reaction on the Y-bearing sperm, which resulted in gender biased semen. Three BALB/c mice were immunized against dairy bull spermatozoa and Freund&...

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Main Authors: Patthanawong W., Pongpiachan P., Mekchay S., Sumretprasong J.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-78650329731&partnerID=40&md5=355b48e361ec5f25016a6b71bc51546a
http://cmuir.cmu.ac.th/handle/6653943832/455
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-4552014-08-29T07:31:47Z Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa Patthanawong W. Pongpiachan P. Mekchay S. Sumretprasong J. The objective of this study was to produce monoclonal antibody (MAb) against Y-bearing spermatozoa. The antibody will be used in cytotoxic immunological reaction on the Y-bearing sperm, which resulted in gender biased semen. Three BALB/c mice were immunized against dairy bull spermatozoa and Freund's complete adjuvant, 106 cells per 100 μl, at two week interval. The spleenocytes, from the mouse that had produced antibodies to the Y-bearing spermatozoa, were used for fusion with the myeloma cells line, X63-Ag8.653, with the aid of polyethylene glycol. It was found, within 14-21 days after fusion, that 148 clones appeared. Three of those clones were capable of binding specifically with white blood cells (WBC) from dairy bulls (n=5) and showed negative response with the WBC from dairy heifers (n=5). The three antibodies were classified to be IgG. One of them, clone number 1F9-3B10, was chosen due to its high antibody activity. The clone was tested by using Immunofluorescent technique with WBC of 20 dairy bulls and 30 dairy heifers, the results showed that 89.5 % of those bull WBC were recognized by the MAb from the clone number 1F9-3B10 (p<0.005). In conclusion, the MAb tends to bind specifically with Y-bearing bull spermatozoa. 2014-08-29T07:31:47Z 2014-08-29T07:31:47Z 2010 Article 3676722 http://www.scopus.com/inward/record.url?eid=2-s2.0-78650329731&partnerID=40&md5=355b48e361ec5f25016a6b71bc51546a http://cmuir.cmu.ac.th/handle/6653943832/455 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The objective of this study was to produce monoclonal antibody (MAb) against Y-bearing spermatozoa. The antibody will be used in cytotoxic immunological reaction on the Y-bearing sperm, which resulted in gender biased semen. Three BALB/c mice were immunized against dairy bull spermatozoa and Freund's complete adjuvant, 106 cells per 100 μl, at two week interval. The spleenocytes, from the mouse that had produced antibodies to the Y-bearing spermatozoa, were used for fusion with the myeloma cells line, X63-Ag8.653, with the aid of polyethylene glycol. It was found, within 14-21 days after fusion, that 148 clones appeared. Three of those clones were capable of binding specifically with white blood cells (WBC) from dairy bulls (n=5) and showed negative response with the WBC from dairy heifers (n=5). The three antibodies were classified to be IgG. One of them, clone number 1F9-3B10, was chosen due to its high antibody activity. The clone was tested by using Immunofluorescent technique with WBC of 20 dairy bulls and 30 dairy heifers, the results showed that 89.5 % of those bull WBC were recognized by the MAb from the clone number 1F9-3B10 (p<0.005). In conclusion, the MAb tends to bind specifically with Y-bearing bull spermatozoa.
format Article
author Patthanawong W.
Pongpiachan P.
Mekchay S.
Sumretprasong J.
spellingShingle Patthanawong W.
Pongpiachan P.
Mekchay S.
Sumretprasong J.
Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa
author_facet Patthanawong W.
Pongpiachan P.
Mekchay S.
Sumretprasong J.
author_sort Patthanawong W.
title Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa
title_short Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa
title_full Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa
title_fullStr Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa
title_full_unstemmed Production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa
title_sort production of monoclonal antibody against male specific antigen on cell membrane of bovine spermatozoa
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-78650329731&partnerID=40&md5=355b48e361ec5f25016a6b71bc51546a
http://cmuir.cmu.ac.th/handle/6653943832/455
_version_ 1681419486448582656