Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase

Background: Purple rice has become a natural product of interest which is widely used for health promotion. This study investigated the preventive effect of purple rice extract (PRE) mixed diet on DMH initiation of colon carcinogenesis. Materials and Methods: Rats were fed with PRE mixed diet one we...

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Main Authors: Ratasark Summart, Teera Chewonarin
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84894647217&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45544
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-455442018-01-24T06:12:02Z Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase Ratasark Summart Teera Chewonarin Background: Purple rice has become a natural product of interest which is widely used for health promotion. This study investigated the preventive effect of purple rice extract (PRE) mixed diet on DMH initiation of colon carcinogenesis. Materials and Methods: Rats were fed with PRE mixed diet one week before injection of DMH (40 mg/kg of body weight once a week for 2 weeks). They were killed 12 hrs after a second DMH injection to measure the level of O 6 -methylguanine and xenobiotic metabolizing enzyme activities. Results: In rats that received PRE, guanine methylation was reduced in the colonic mucosa, but not in the liver, whereas PRE did not affect xenobiotic conjugation, with reference to glutathione-S-transferase or UDP-glucuronyl transferase. After 5 weeks, rats that received PRE with DMH injection had fewer ACF in the colon than those treated with DMH alone. Interestingly, a PRE mixed diet inhibited the activity of bacterial β-glucuronidase in rat feces, a critical enzyme for free methylazoxymethanol (MAM) release in the rat colon. These results indicated that purple rice extract inhibited β-glucuronidase activity in the colonic lumen, causing a reduction of MAM-induced colonic mucosa DNA methylation, leaded to decelerated formation of aberrant crypt foci in the rat colon. Conclusions: The supplemented purple rice extract might thus prevent colon carcinogenesis by the alteration of the colonic environment, and thus could be further developed for neutraceutical products for colon cancer prevention. 2018-01-24T06:12:02Z 2018-01-24T06:12:02Z 2014-01-01 Journal 2476762X 15137368 2-s2.0-84894647217 10.7314/APJCP.2014.15.2.749 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84894647217&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/45544
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Background: Purple rice has become a natural product of interest which is widely used for health promotion. This study investigated the preventive effect of purple rice extract (PRE) mixed diet on DMH initiation of colon carcinogenesis. Materials and Methods: Rats were fed with PRE mixed diet one week before injection of DMH (40 mg/kg of body weight once a week for 2 weeks). They were killed 12 hrs after a second DMH injection to measure the level of O 6 -methylguanine and xenobiotic metabolizing enzyme activities. Results: In rats that received PRE, guanine methylation was reduced in the colonic mucosa, but not in the liver, whereas PRE did not affect xenobiotic conjugation, with reference to glutathione-S-transferase or UDP-glucuronyl transferase. After 5 weeks, rats that received PRE with DMH injection had fewer ACF in the colon than those treated with DMH alone. Interestingly, a PRE mixed diet inhibited the activity of bacterial β-glucuronidase in rat feces, a critical enzyme for free methylazoxymethanol (MAM) release in the rat colon. These results indicated that purple rice extract inhibited β-glucuronidase activity in the colonic lumen, causing a reduction of MAM-induced colonic mucosa DNA methylation, leaded to decelerated formation of aberrant crypt foci in the rat colon. Conclusions: The supplemented purple rice extract might thus prevent colon carcinogenesis by the alteration of the colonic environment, and thus could be further developed for neutraceutical products for colon cancer prevention.
format Journal
author Ratasark Summart
Teera Chewonarin
spellingShingle Ratasark Summart
Teera Chewonarin
Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase
author_facet Ratasark Summart
Teera Chewonarin
author_sort Ratasark Summart
title Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase
title_short Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase
title_full Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase
title_fullStr Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase
title_full_unstemmed Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase
title_sort purple rice extract supplemented diet reduces dmh-induced aberrant crypt foci in the rat colon by inhibition of bacterial β-glucuronidase
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84894647217&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45544
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