Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805

Microbial transformation of chlormadinone acetate to delmadinone acetate was investigated using free and immobilized cells of Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805 in liquid-liquid biphasic system and a liposomal medium. For liquid-liquid biphasic system, n-decane, n-octa...

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Main Authors: Manosroi J., Sripalakit P., Manosroi A.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-0142075804&partnerID=40&md5=923c85d822e52268307efeff4506e889
http://cmuir.cmu.ac.th/handle/6653943832/4556
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-45562014-08-30T02:42:35Z Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805 Manosroi J. Sripalakit P. Manosroi A. Microbial transformation of chlormadinone acetate to delmadinone acetate was investigated using free and immobilized cells of Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805 in liquid-liquid biphasic system and a liposomal medium. For liquid-liquid biphasic system, n-decane, n-octanol, chloroform and butyl acetate were used as organic solvents. In liposomal medium, chlormadinone acetate was entrapped in liposomes composed of phosphatidylcholine and cholesterol. For both immobilized and free suspension biocatalysts, yields were higher in the aqueous medium compared to in liquid-liquid biphasic media. In all cases, the highest yields were obtained in the aqueous system. For the aqueous medium, a product yield of ∼48% was attained at 3h using the free suspension A. simplex cells. The aqueous medium afforded ∼47% product yield at 48h when freely suspended B. sphaericus was used. © 2003 Elsevier Inc. All rights reserved. 2014-08-30T02:42:35Z 2014-08-30T02:42:35Z 2003 Article 01410229 10.1016/S0141-0229(03)00161-3 EMTED http://www.scopus.com/inward/record.url?eid=2-s2.0-0142075804&partnerID=40&md5=923c85d822e52268307efeff4506e889 http://cmuir.cmu.ac.th/handle/6653943832/4556 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Microbial transformation of chlormadinone acetate to delmadinone acetate was investigated using free and immobilized cells of Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805 in liquid-liquid biphasic system and a liposomal medium. For liquid-liquid biphasic system, n-decane, n-octanol, chloroform and butyl acetate were used as organic solvents. In liposomal medium, chlormadinone acetate was entrapped in liposomes composed of phosphatidylcholine and cholesterol. For both immobilized and free suspension biocatalysts, yields were higher in the aqueous medium compared to in liquid-liquid biphasic media. In all cases, the highest yields were obtained in the aqueous system. For the aqueous medium, a product yield of ∼48% was attained at 3h using the free suspension A. simplex cells. The aqueous medium afforded ∼47% product yield at 48h when freely suspended B. sphaericus was used. © 2003 Elsevier Inc. All rights reserved.
format Article
author Manosroi J.
Sripalakit P.
Manosroi A.
spellingShingle Manosroi J.
Sripalakit P.
Manosroi A.
Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805
author_facet Manosroi J.
Sripalakit P.
Manosroi A.
author_sort Manosroi J.
title Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805
title_short Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805
title_full Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805
title_fullStr Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805
title_full_unstemmed Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805
title_sort biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized arthrobacter simplex atcc 6946 and bacillus sphaericus atcc 13805
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-0142075804&partnerID=40&md5=923c85d822e52268307efeff4506e889
http://cmuir.cmu.ac.th/handle/6653943832/4556
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