Factors affecting the biotransformation of chlormadinone acetate to delmadinone acetate

• Main Authors: Manosroi J., Sripalakit P., Manosroi A.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-0037678458&partnerID=40&md5=8ee7b0e8249226399c23b6cb6aaf0486; http://cmuir.cmu.ac.th/handle/6653943832/4557
• Institution: Chiang Mai University

Factors affecting the aqueous biotransformation of chlormadinone acetate to delmadinone acetate by freely suspended cells of Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805 were investigated. The effects of exogenous electron carrier (menadione), cosolvent (dimethylformamide), steroid inducer (hydrocortisone), surfactant (Tween 80) and alternative carbon source (D-(+)-glucose), on the production yield of delmadinone acetate were identified. The biotransformations were performed in shaken flasks at 25±2°C for 72h. The optimal conditions for the biotransformation with A. simplex were: 0.25mM chlormadinone acetate, 5% DMF, 0.41mM hydrocortsione and 0.75% (w/v) Tween 80. With B. sphaericus, the optimal conditions were: 0.6mM menadione, 0.12mM chlormadione acetate, 5% DMF, 0.41mM hydrocortisone and 0.25gdm-3 D-(+)-glucose. Under optimal conditions, the product yields were 28.7 and 36.9% for A. simplex and B. sphaericus biotransformations, respectively. © 2003 Elsevier Science B.V. All rights reserved.