Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column

The use of a small guard column (5-mm length) as a miniature analytical column in low-pressure liquid chromatography based on a sequential injection with lab-on-valve system was demonstrated. A strong initial mobile phase was used to rapidly deliver the sample zone to the column prior to simultaneou...

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Main Authors: Supaporn Kradtap Hartwell, Alison Kehling, Somchai Lapanantnoppakhun
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904751009&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45580
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-455802018-01-24T06:12:36Z Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column Supaporn Kradtap Hartwell Alison Kehling Somchai Lapanantnoppakhun The use of a small guard column (5-mm length) as a miniature analytical column in low-pressure liquid chromatography based on a sequential injection with lab-on-valve system was demonstrated. A strong initial mobile phase was used to rapidly deliver the sample zone to the column prior to simultaneous separation of analytes with weaker mobile phase. Separation of methyl-, ethyl-, propyl-, and butyl-4-hydroxybenzoates (MP, EP, PP, and BP) was achieved within about 120 s with sufficient peak resolution (all higher than 1.3) using 2,500 μL of mobile phase (composed of 1,087 μL of MeOH) per run. Detection limits were found to be 6.5, 8.0, 0.5, and 0.6 μmol L -1 for MP, EP, PP, and BP, respectively. The system was tested with common commercially available skin lotions and wet wipe products. The analysis results were in good agreement with those obtained from the high-performance liquid chromatography with a 10-cm-length packed column. © 2014 Springer-Verlag. 2018-01-24T06:12:36Z 2018-01-24T06:12:36Z 2014-01-01 Journal 16121112 00095893 2-s2.0-84904751009 10.1007/s10337-014-2697-6 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904751009&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/45580
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description The use of a small guard column (5-mm length) as a miniature analytical column in low-pressure liquid chromatography based on a sequential injection with lab-on-valve system was demonstrated. A strong initial mobile phase was used to rapidly deliver the sample zone to the column prior to simultaneous separation of analytes with weaker mobile phase. Separation of methyl-, ethyl-, propyl-, and butyl-4-hydroxybenzoates (MP, EP, PP, and BP) was achieved within about 120 s with sufficient peak resolution (all higher than 1.3) using 2,500 μL of mobile phase (composed of 1,087 μL of MeOH) per run. Detection limits were found to be 6.5, 8.0, 0.5, and 0.6 μmol L -1 for MP, EP, PP, and BP, respectively. The system was tested with common commercially available skin lotions and wet wipe products. The analysis results were in good agreement with those obtained from the high-performance liquid chromatography with a 10-cm-length packed column. © 2014 Springer-Verlag.
format Journal
author Supaporn Kradtap Hartwell
Alison Kehling
Somchai Lapanantnoppakhun
spellingShingle Supaporn Kradtap Hartwell
Alison Kehling
Somchai Lapanantnoppakhun
Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column
author_facet Supaporn Kradtap Hartwell
Alison Kehling
Somchai Lapanantnoppakhun
author_sort Supaporn Kradtap Hartwell
title Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column
title_short Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column
title_full Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column
title_fullStr Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column
title_full_unstemmed Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column
title_sort low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904751009&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45580
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